Correction to: Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by
- PDF / 433,460 Bytes
- 2 Pages / 595.276 x 790.866 pts Page_size
- 1 Downloads / 158 Views
www.virosin.org www.springer.com/12250
(0123456789().,-volV)(0123456789().,-volV)
CORRECTION
Correction to: Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference Yafei Zhang1,2 • Changtai Wang1,2 • Mingfeng Han3 • Jun Ye1,2 • Yong Gao3 • Zhongping Liu1,2 Tengfei He1,2 • Tuantuan Li3 • Mengyuan Xu1,2 • Luping Zhou1,2 • Guizhou Zou1,2 • Mengji Lu4 Zhenhua Zhang1,2
• •
Ó Wuhan Institute of Virology, CAS 2020
Correction to: Virologica Sinica (2020) https://doi.org/10.1007/s12250-020-00273-8
In the original version of Fig. 4I and 4J, the cutoff lines were accidently shifted during figure layout. Figures 4I and 4J are re-drawn and provided below.
Yafei Zhang, Changtai Wang, Mingfeng Han, Jun Ye and Yong Gao contributed equally to this work. The original article can be found online at https:// doi.org/10.1007/s12250-020-00273-8. & Zhenhua Zhang [email protected] & Mengji Lu [email protected] 1
Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei 230601, China
2
Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei 230601, China
3
Department of Internal Medicine, The Second Hospital of Fuyang, Fuyang 236015, China
4
Institute for Virology, University Hospital of Essen, University of Duisburg-Essen, 45147 Essen, Germany
123
Proportion of sputums (%)
A
100
B
n = 109
80 60
47.7%
40
29.4%
22.9%
20 0%
0 + ORF 1ab + N region + + SARS-CoV-2 RNA in sputums
C
Proportion of throat swabs (%)
Virologica Sinica
100
n = 72
80 60
52.8%
40
26.4%
20
0 + ORF 1ab + N region + + SARS-CoV-2 RNA in throat swabs
D
E
P
P
F
P
G
H
P
P
RPP30 RNA in sputums (Ct values)
n= 28
52
57
P < 0.0001
84
25 P < 0.0001
26 24 22 20 18 16 Pos
Neg ORF 1ab
Pos
Neg N region
SARS-CoV-2 RNA in sputums
J
n=
RPP30 RNA in throat swabs (Ct values)
I
20.8%
0%
P
15
30
57 P < 0.0001
34
38
P < 0.0001
28
26
24
22 Pos
Neg ORF 1ab
Pos
Neg N region
SARS-CoV-2 RNA in throat swabs
Fig. 4 The RT-PCR detection of SARS-CoV-2 RNAs in 181 sputum and throat swab specimens from 20 patients with conflicting RT-PCR results. 109 paired sputum (A) and 72 throat swab (B) specimens were collected and subjected to SARS-CoV-2 specific RT-PCR assays targeting the ORF1ab and N regions. ?: RT-PCR positive; -: RT-PCR negative. Pearson correlation coefficients for the levels of detected SARS-CoV-2 RNA (based on the assays for ORF1ab and N region) and RPP30 RNAs in patient samples were calculated. For sputum specimens: C ORF1ab and N region; D ORF1ab and RPP30; E N region and RPP30. For throat swab specimens: F ORF1ab and N region; G ORF1ab and RPP30; H N region and RPP30. The Ct values of RPP30 RT-PCR were used to analyze the positive and negative results of ORF1ab and N-specific RT-PCR assays with 109 sputum (I) and 72 throat swab (J) specimens. Pos: positive results; Neg: negative results. The negative RT-PCR results of SARS-CoV-2 detection correspond to high Ct value
Data Loading...
