Cyclodextrin subject-object recognition-based aptamer sensor for sensitive and selective detection of tetracycline
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ORIGINAL PAPER
Cyclodextrin subject-object recognition-based aptamer sensor for sensitive and selective detection of tetracycline Yao-hui Wu 1 & Hao Bi 1 & Ge Ning 2 & Zheng-gang Xu 1,3 & Gao-qiang Liu 1 & Yong-hong Wang 1 & Yun-lin Zhao 1 Received: 14 November 2019 / Revised: 30 June 2020 / Accepted: 2 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Based on a β-cyclodextrin (β-CD) subject-object competition model, a simple and sensitive electrochemical aptamer sensor for the determination of tetracycline (TET) was fabricated. First, the TET aptamer modified with ferrocene (Fc) as a signal molecule was captured by β-CD loaded onto the gold electrode. Subsequently, TET was added to the detection system, causing a binding event between the target and aptamer with strong affinity during which process the aptamer configuration was changed from the original upright linear state to an agglomeration structure, resulting in its departure from the electrode surface. Consequently, an “off-signal” was turned along with the presence of the target. The results indicated that the TET concentration had a linear response to the signal ranging from 0.01 to 100 nM and the accurate detection limit could reach as low as 0.008 nM (3δ). The fabricated TET biosensor also showed outstanding detection specificity. Moreover, the suitability of the developed method was demonstrated in the determination of TET concentrations in different samples comprising water, milk, and bacteria culture medium, achieving acceptable recoveries for spiked samples ranging from 96.0 to 104.4%. This detection system was simple, economical, time-saving but remarkably sensitive, selective, and efficient, potentially rendering services in food safety screening and medical testing. Keywords TET . Aptamer . Subject-object recognition . β-Cyclodextrin
Introduction Antibiotics are broadly used in safeguarding human and animal health from bacterial infections. However, they were under increasing threat from overuse. For example, excessive use of TET in medicine as an antibiotic and growth promoter might lead to adverse side effects on human health and the environment [1]. It heavily disrupted the ecology of the human microbiome (the collection of cells, genes, and metabolites of bacteria, eukaryotes, and viruses that inhabit the human body) and brought about the development of bacterial resistance [2–4]. According to relative statistics, Europe and the USA suffered over 50,000 deaths due * Yong-hong Wang [email protected]; [email protected] 1
College of Life Science and Technology, Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China
2
International Education Institute, Hunan University of Chinese Medicine, Changsha 410208, China
3
Hunan Urban and Rural Ecological Planning and Restoration Engineering Research Center, Hunan City University, Yiyang, China
to antibiotic-resistant pathogens and the figures for the whole world are projected to rise to 1
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