Development of forcespun fiber-aligned scaffolds from gelatin-zein composites for potential use in tissue engineering an
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Research Letter
Development of forcespun fiber-aligned scaffolds from gelatin–zein composites for potential use in tissue engineering and drug release Narsimha Mamidi, Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México Irasema Lopez Romo, Tecnológico de Monterrey, Centro de Biotecnología-FEMSA, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, N.L., C.P. 64849, México Héctor Manuel Leija Gutiérrez, Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México Enrique V. Barrera, Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México; Department of Materials Science and NanoEngineering, Rice University, Houston, TX 77005, USA; Department of Chemistry, Rice University, Houston, TX 77005, USA Alex Elías-Zúñiga, Tecnológico de Monterrey, Campus Monterrey, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col Tecnológico C.P. 64849, Monterrey, Nuevo León, México Address all correspondence to Narsimha Mamidi at [email protected] (Received 14 November 2017; accepted 25 April 2018)
Abstract In this study, based on the collection process, three-dimensional aligned fiber scaffolds from gelatin and zein protein were manufactured using Forcespinning®. The homogeneous blending of gelatin:zein (1:4) showed improved tensile and good hydrophobic properties (water contact angle of 115 °C). Cell viability, adhesion, proliferation, and drug release were measured. The cell viability was studied with human fibroblasts and a low cytotoxic effect was observed. Berberine drug release was measured and sustained release rate was observed over 15 days. The morphologic features, prolonged drug release, and cytotoxicity results suggest that these fibers could be appropriate for drug delivery and tissue engineering applications.
Introduction The extracellular matrix (ECM) provides support for cells and creates an environment where it influences cell growth, shape, and differentiation.[1] Bio-fabrication and the design of engineered fiber scaffolds has been inspired by the natural structure of ECM. The behavior of cell lines, including differentiation, cell adhesion, proliferation, and migration, is influenced by surface topographic properties. Soft lithography, inkjet printing, and photochemistry methods are utilized to construct a surface with specific topographic properties.[2] Unidirectional ordered patterns showed great interest in the collagen fibers and the cells found in tendon, ligament, and muscle due to their similarities to native ECM–cell systems.[3,4] Based on the fiber rearrangement, random and aligned fibers are used to create structures that contained ECM-mimicking morphologies.[5] Fiber scaffolds can be manufactured with micrometer to nanometer diameter range. Self-as
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