Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock
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BioMed Central
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Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein Larissa Lumi Watanabe Ishikawa1, Thaís Graziela Donegá França1, Fernanda Chiuso-Minicucci1, Sofia Fernanda Gonçalves Zorzella-Pezavento1, Nelson Mendes Marra2, Paulo Câmara Marques Pereira3, Célio Lopes Silva4 and Alexandrina Sartori*1 Address: 1Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), Botucatu, São Paulo, 18618000, Brazil, 2Department of Parasitology, Biosciences Institute, São Paulo State University (UNESP), Botucatu, São Paulo, 18618-000, Brazil, 3Department of Tropical Diseases, Medical School, São Paulo State University (UNESP), Botucatu, São Paulo, 18618-000, Brazil and 4Department of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, São Paulo, 14049-900, Brazil Email: Larissa Lumi Watanabe Ishikawa - [email protected]; Thaís Graziela Donegá França - [email protected]; Fernanda Chiuso-Minicucci - [email protected]; Sofia Fernanda Gonçalves Zorzella-Pezavento - [email protected]; Nelson Mendes Marra - [email protected]; Paulo Câmara Marques Pereira - [email protected]; Célio Lopes Silva - [email protected]; Alexandrina Sartori* - [email protected] * Corresponding author
Published: 16 July 2009 Genetic Vaccines and Therapy 2009, 7:11
doi:10.1186/1479-0556-7-11
Received: 5 May 2009 Accepted: 16 July 2009
This article is available from: http://www.gvt-journal.com/content/7/1/11 © 2009 Ishikawa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Protein-calorie malnutrition (PCM) is the most common type of malnutrition. PCM leads to immunodeficiency and consequent increased susceptibility to infectious agents. In addition, responses to prophylactic vaccines depend on nutritional status. This study aims to evaluate the ability of undernourished mice to mount an immune response to a genetic vaccine (pVAXhsp65) against tuberculosis, containing the gene coding for the heat shock protein 65 from mycobacteria. Methods: Young adult female BALB/c mice were fed ad libitum or with 80% of the amount of food consumed by a normal diet group. We initially characterized a mice model of dietary restriction by determining body and spleen weights, hematological parameters and histopathological changes in lymphoid organs. The ability of splenic cells to produce IFN-gamma and IL-4 upon in vitro stimulation with LPS or S. aureus and the serum titer of specific IgG1 and IgG2a anti-hsp65 antibodies after intramuscular immunization with pVAXhsp65 was then tested. Results: Dietary restriction significantly decreased body and spleen weights and also the total lymphocyte count in bloo
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