Does the Addition of Poly(glycolide-co-lactide) to Teicoplanin-Containing Poly(methyl methacrylate) Beads Change the Elu
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ORIGINAL ARTICLE
Does the Addition of Poly(glycolide‑co‑lactide) to Teicoplanin‑Containing Poly(methyl methacrylate) Beads Change the Elution Characteristics? Seyran Kilinç1 · Özhan Pazarci1 · Neşe Keklikcioğlu Çakmak2 · Ayça Taş3 Received: 25 February 2020 / Accepted: 10 April 2020 © Indian Orthopaedics Association 2020
Abstract Background The objective of our study was to measure and compare the elution characteristics of teicoplanin from poly(methyl methacrylate) PMMA beads with those of poly(glycolide-co-lactide) PGLA-added beads. Methods The study included two groups of PMMA + teicoplanin beads. PMMA was added to teicoplanin in Group 1 and PMMA + PGLA was added to teicoplanin in Group 2. A total of 16 beads of 1 cm3 were created for each group. Samples were added individually to tubes containing 3 ml of phosphate-buffered saline (PBS). Antibiotic elution was measured by measuring absorbance values of 1-ml samples taken at regular intervals using a UV–Vis spectrophotometer and cumulative percentages of drug release were calculated. In addition, the spectra of teicoplanin were identified using a FTIR spectrophotometer in a wavelength range of 400–4000 cm−1. Results Drug elution in the PBS medium was measured and compared for Groups 1 and 2. The cumulative percentage of drug release from the PGLA-added beads (Group 2) was significantly higher (p = 0.01). The molecular structure of teicoplanin was also confirmed using FTIR. Conclusion The in vitro results showed that the addition of biodegradable PGLA into bone cement functions as a watersoluble porogen which allows for significant increases in the elution of teicoplanin from cement. This increase in elution suggests that the PGLA would allow for further fluid contact and exchange with the previously entrapped drug. These results may have important clinical applications. Keywords Teicoplanin · Glycolide-co-lactide · Cement spacer · Elution · Polymethylmethacrylate
Introduction Thanks to modern surgical techniques and antibiotic prophylaxes, orthopedic infections are less common, yet remain a challenge for orthopedists [1]. Debridement and systemic antibiotics chosen based on culture results are used in the treatment of orthopedic infections. However, because of * Seyran Kilinç [email protected] 1
Department of Orthopedics and Traumatology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, Turkey
2
Department of Chemical Engineering, Faculty of Engineering, Sivas Cumhuriyet University, 58140 Sivas, Turkey
3
Department of Nutrition and Dietetics, Faculty of Health Sciences, Sivas Cumhuriyet University, Sivas, Turkey
the bacterial biofilms formed during the infection stage, antibiotic efficacy cannot be achieved in the infected tissues, which may interfere with effective treatment [2]. High concentrations of antibiotics in the infected area cannot be achieved through intravenous administration but instead through the placement of antibiotic-added bone cement in the infection site [3, 4]. Although this method has been in use
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