Dopamine and norepinephrine assistant-synthesized nanoflowers immobilized membrane with peroxidase mimic activity for ef
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ORIGINAL ARTICLE
Dopamine and norepinephrine assistant‑synthesized nanoflowers immobilized membrane with peroxidase mimic activity for efficient detection of model substrates Seyma Dadi1 · Cagla Celik1 · Amit K. Mandal2 · Ismail Ocsoy1 Received: 13 July 2020 / Accepted: 1 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Herein, we prepared dopamine assistant-synthesized nanoflowers (dNFs) and norepinephrine assistant-synthesized nanoflowers (neNFs) as novel nano-biocatalysts with intrinsic and effective peroxidase mimic activities. The NFs dispersed in solution or immobilized on the filter membrane served as the Fenton reagent in the presence of hydrogen peroxide ( H2O2) allowing spectrophotometric and colorimetric detection of m-cresol and dopamine. Due to its high polar surface property and porosity, the neNFs exhibited remarkably high catalytic activity than dNFs in both solution or in immobilized states. The activity performance of neNFs as a function of varied concentrations of neNFs or m-cresol or dopamine or H 2O2; and reaction time was also studied. The oxidative reaction of 0.5 mM m-cresol (in the presence of 4-AAP and H 2O2) or 0.25 mM dopamine (in the presence of H2O2) catalyzed by the neNFs (1 mg/mL) were visually detected within 10 min in solution. Whereas, after immobilization on filter membrane, the colorimetric detection of the same concentrations of m-cresol or dopamine was achieved in 30 min using neNFs (2 mg/mL). Furthermore, the effect of repeated use of the neNFs in solution or immobilized states on the catalytic activity was also investigated. We expect that, the NFs formed from non-enzyme molecules possess peroxidase mimic activities that could imply to a study for bioanalytical and biomedical application in the near future. Keywords Dopamine nanoflowers · Norepinephrine nanoflowers · Fenton reagent · Peroxidase mimic activities
Introduction The synthesis of enzyme-inorganic nanoflowers (NFs) setoff an encouraging development among enzyme immobilization methods owing to its superb catalytic activities and stabilities (Ge et al. 2012). The mechanism of the formation of NFs in phosphate-buffered saline (PBS) relies on the coordination reaction between amide groups in the protein backbone and copper (II) ions (Cu2+). Initially, copper phosphate nanocrystals (Cu3(PO4)2) are formed as seeds and the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13204-020-01577-7) contains supplementary material, which is available to authorized users. * Ismail Ocsoy [email protected] 1
Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey
Chemical Biology Laboratory, Centre for Nanotechnology Sciences, Raiganj University, 733134 Raiganj, India
2
enzymes bind to these nanocrystals through self-assembly to produce flower-shaped hybrid structures. Since the discovery of the NFs, various enzymes and metal ions under different experimental conditions have been utili
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