Effect of Photomodulation Therapy on Development of Oxidative Stress in Blood Leukocytes of Rats with Streptozocin-Induc
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ct of Photomodulation Therapy on Development of Oxidative Stress in Blood Leukocytes of Rats with Streptozocin-Induced Diabetes Mellitus O. I. Karmasha, *, M. Ya. Liutaa, A. M. Korobovb, and N. O. Sybirnaa aDepartment
of Biology, Ivan Franko Lviv National University, Lviv, Ukraine Laboratory of Quantum Biology and Quantum Medicine, Karazin Kharkiv National University, Kharkiv, Ukraine *e-mail: [email protected]
b
Received December 6, 2019; revised February 19, 2020; accepted September 18, 2020
Abstract—Oxidative stress is among the main causes of developing severe complications in diabetes mellitus (DM). Existing pharmaceuticals, although efficient in reducing the blood glucose level, infrequently demonstrate antioxidant properties. On the other hand, there are many reliable reports about a broad range of biological activity exhibited by photobiomodulation therapy (PBMT). Its potential sugar-lowering and antioxidant action makes this type of therapy a promising option for the treatment of DM and its complications. The effect of PBMT on the state of the antioxidant defense system in blood leukocytes was investigated in rats with streprozocin-induced DM. This study has shown that the PBMT increased superoxide dismutase (SOD) activity in rats with DM and normalized the content of oxidative stress markers (thiobarbituric acid (TBA)-active products, oxidatively modified proteins, and protein glycation end products). Keywords: diabetes mellitus, photobiomodulation therapy, blood leukocytes, oxidative stress, antioxidant protection system DOI: 10.3103/S0095452720050114
INTRODUCTION Diabetes mellitus is a severe chronic disease and, at the same time, is one of the most widespread chronic pathological conditions. It is characterized by uncontrollable hyperglycemia, which emerges due to the shortage of insulin or insulin-resistance in the peripheral tissues of the body. A chronic blood glucose increase typical of DM causes a significant group of severe complications, such as nephropathy, cardiomyopathy, and retinopathy. But the main cause of diabetic complications is the concomitant development of oxidative stress through overproduction of reactive oxygen species (ROS) capable to ruin cells, especially their membrane structures [1]. Photobiomodulation therapy (PBMT) has been known for almost 50 years but has not been widely applied due to insufficient knowledge about the molecular and cellular mechanisms of its action. At the same time, PBMT is practiced as a component of physiotherapeutic treatment methods in many diseases and applied widely around the world. PBMT is based on different light sources (lasers and photodiodes) and different spectra in visible and infrared ranges. Apart from this, there are many reports confirming the presence of biological activity of PBMT. It has been shown that PBMT actively affects cell proliferation (fibroblasts and keratinocytes) and wound
healing [2] and activates the adenosine triphosphate (ATP) production by mitochondria [3]. The key point in the question about the use of PBMT
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