Effects of Selenium Intervention on Chronic Fluorosis-Induced Renal Cell Apoptosis in Rats
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Effects of Selenium Intervention on Chronic Fluorosis-Induced Renal Cell Apoptosis in Rats Shu-ying Yang & Lei Zhang & Ke-ke Miao & Wei Qian & Zi-gui Zhang
Received: 12 March 2013 / Accepted: 18 March 2013 / Published online: 17 April 2013 # Springer Science+Business Media New York 2013
Abstract This study aims to explore the effect of selenium in fluoride-induced renal cell apoptosis in rats and determine the optimal level of selenium in drinking water to prevent fluorosis. Experimental animals were divided into a control group, a sodium fluoride-treated group (NaF, 50 mg/L), three sodium selenite-treated groups (Na2SeO3, 0.375, 0.75, and 1.5 mg/L), and three selenium + NaF-treated groups (Na2SeO3, 0.375, 0.75, and 1.5 mg/L; NaF, 50 mg/L). Ultrastructural changes in the kidney tissues of each group were observed by transmission electron microscopy. Apoptosis was detected by terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling assay and the expressions of Bcl-2 and Bax proteins were detected by immunohistochemical methods. The expressions of Bcl-2 and Bax mRNA were detected by reverse transcriptionpolymerase chain reaction. The results showed that Bcl-2, Bax, and Bax/Bcl-2 protein expressions in the fluoride and high selenium groups were highly elevated compared with the control group (P
