Efficacy of Gefitinib Combined with 125 I Radioactive Particles in the Treatment of Transplanted Lung Cancer Tumors in N
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LABORATORY INVESTIGATION
INTERVENTIONAL ONCOLOGY
Efficacy of Gefitinib Combined with 125I Radioactive Particles in the Treatment of Transplanted Lung Cancer Tumors in Nude Mice Chaojie Li1 • Linyan Yao1 • Ju Gong1 • Haopeng Pang1 • Qungang Shan1 Ziyin Wang1 • Jian Lu1 • Zhongmin Wang1
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Received: 28 March 2020 / Accepted: 30 May 2020 Springer Science+Business Media, LLC, part of Springer Nature and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2020
Abstract Objective To investigate the efficacy of gefitinib combined with iodine-125 (125I) radioactive particles in the treatment of transplanted tumors of the lung cancer cell line A549 in nude mice. Materials and Methods Twenty-four nude mice were inoculated with A549-luc human lung adenocarcinoma cells stably expressing luciferase. The tumor size was approximately 8–10 mm after 20 days. The mice were randomly divided into four groups: a control group (n = 6), an 125I particles group (n = 6), a gefitinib group (n = 6) and a gefitinib combined 125I particles implantation group (n = 6). Tumor growth was observed, and changes in tumor size were continuously measured. Bioluminescence imaging was used to detect the bioluminescence activity of human lung adenocarcinoma A549-luc cells containing the luciferase reporter gene in vivo. After 35 days, the nude mice were sacrificed, and a tumor growth curve was drawn. Results Before treatment, the tumor volumes of the four groups were not significantly different. The tumor volume difference was statistically significant in the four groups (control group, 125I radioactive particles, gefitinib group and combined drug group) at 5 weeks after treatment
(F = 10.305, P \ 0.05). The tumor size in the gefitinib combined with 125I particles group was significantly smaller than that in the gefitinib, 125I particles and control groups and significantly smaller than that before treatment. There was no significant difference in the bioluminescence signal intensity between the four groups before treatment. The numbers of biofluorescence photons difference were statistically significant in the four groups (F = 28.975, P \ 0.05). The bioluminescence signal intensity in the gefitinib combined with 125I particles group was significantly lower than that in the 125I particles, gefitinib and control groups and significantly lower than that before treatment. Conclusion Gefitinib combined with 125I radioactive particles brachytherapy can significantly inhibit tumor growth. Keywords 125I radioactive particles Lung adenocarcinoma Gefitinib Biofluorescence imaging 18fluorine-fluorinedeoxyglucose (18F-FDG)
Introduction Chaojie Li, Linyan Yao and Ju Gong have contributed equally to this work. & Jian Lu [email protected] & Zhongmin Wang [email protected] 1
Department of Radiology, Ruijin Hospital/Luwan Branch, Shanghai Jiao Tong University School of Medicine, No.197, Ruijin 2nd Road, Huangpu District, Shanghai 200020, China
Cancer is a major public health problem worldwide, and according to global cancer st
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