Efficient bioconversion of sugarcane tops biomass into biofuel-ethanol using an optimized alkali-ionic liquid pretreatme
- PDF / 1,488,853 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 16 Downloads / 267 Views
ORIGINAL ARTICLE
Efficient bioconversion of sugarcane tops biomass into biofuel-ethanol using an optimized alkali-ionic liquid pretreatment approach Vishal Sharma 1 & Parushi Nargotra 1 & Surbhi Sharma 1 & Bijender Kumar Bajaj 1 Received: 27 August 2020 / Revised: 27 October 2020 / Accepted: 29 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Sugarcane tops (SCT) may be an imperative lignocellulosic feedstock for production of renewable energy in view of ever-rising global energy demands. The current study presents the first ever report of combinative pretreatment of sugarcane tops biomass using ionic liquid (1-ethyl-3-methylimidazolium chloride) and alkali (ammonium carbonate) for effective and enhanced saccharification. The enzymatic hydrolysis of combinatorially pretreated SCT biomass resulted in a significantly higher reducing sugar yield (172.34 mg/g biomass) as compared to that obtained after standalone IL (85.9 mg/g biomass) or alkali pretreatment (102.6 mg/g biomass). The saccharification enzymes (cellulase/xylanase) used for hydrolysis of pretreated SCT biomass were in-house produced from an IL-tolerant, newly isolated fungus Penicillium chrysogenum VS4. Optimization of process variables for combined pretreatment was accomplished based on design of experiments, and enhanced reducing sugar yield was obtained. The experimental design for ascertaining optimal level of process variables, i.e., biomass loading (5.33%, w/w), temperature (100 °C), and time (4.50 h) was validated. Optimization of the process parameters resulted in 25.27% increased reducing sugar yield (215.89 mg/g biomass) as compared to that under unoptimized process. Ultrastructural analysis of SCT biomass after combined pretreatment was investigated by scanning electron microscopy and Fourier transform infrared spectroscopy which indicated that the biomass had undergone drastic alterations and substantial disintegration due to pretreatment. The current study highlights the potential of combinative pretreatment strategy for efficient conversion of SCT biomass. Such combined pretreatment approaches may be extended to other biomass feedstocks as well for developing successful biorefineries. Keywords Bioethanol . Optimization . Penicillium chrysogenum VS4 . Pretreatment . Sugarcane tops
1 Introduction Fossil fuel depletion rate coupled with deep environmental concerns serve as catalysts for intensification of research in exploring and developing renewable and sustainable sources of energy [1]. Among various renewable alternatives, lignocellulosic biomass (agricultural and forestry residues, energy crops, municipal solid waste, and others) represents a promising, profusely accessible, cost-effective, and sustainable energy resource for the future [2, 3]. Lignocellulosic biomass (LB) is constituted of mainly cellulose, hemicellulose, lignin, ash, and other extractives, in which the thickness and composition
* Bijender Kumar Bajaj [email protected] 1
School of Biotechnology, University of Jammu, Jammu, Ind
Data Loading...
