Electrochemiluminescence Analysis of Hydrogen Peroxide Using L012 Modified Electrodes
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ORIGINAL PAPER
Electrochemiluminescence Analysis of Hydrogen Peroxide Using L012 Modified Electrodes Yuling Wang1 · Dechen Jiang1 · Hong‑Yuan Chen1 Received: 9 April 2020 / Accepted: 15 May 2020 / Published online: 26 May 2020 © The Nonferrous Metals Society of China 2020
Abstract In this paper, a luminol analog, L012, with a high-electrochemiluminescence (ECL) illumination is covalently assembled at indium tin oxide (ITO) surface that exhibits the ECL response to hydrogen peroxide in the solution. The ITO slide is firstly functioned with amine group that react with glutaraldehyde to introduce aldehyde group at the surface. Upon the exposure to L012 with amine group, the reaction between the aldehyde group and the amine group results in the linkage of L012 at the electrode surface. In the presence of hydrogen peroxide, enhanced ECL from L012 at the electrode surface is observed that is linearly related with the concentration of hydrogen peroxide. The detection limit is determined to be 4.3 μM (S/N = 3). The successful establishment of ECL response to hydrogen peroxide using L012 modified electrode will provide a new functioned ECL surface for the future ECL imaging of hydrogen peroxide release from single cells. Keywords Electrochemiluminescence · L012 · High ECL illumination · Functioned surface · Hydrogen peroxide
1 Introduction The analysis of small molecules in biological samples is critical to understand their functions associated with cellular heterogeneity and disease states [1–3]. For example, natural metabolic byproduct of oxygen, such as hydrogen peroxide (H2O2), plays a significant role within cells and between cells as a signaling and regulating molecule [4, 5]. In addition, the concentration of hydrogen peroxide is a weighty biological parameter because it is the main product of metabolism in the human body [6] resulting in the oxidative stress [7, 8]. High levels of oxidative stress caused by intracellular hydrogen peroxide and some other reactiveoxygen species may damage DNA and contribute to cancer [9]. Therefore, the quantitative analysis of hydrogen peroxide is significant for the study in oxidative stress, neurodegenerative disease, cancer, etc.[10, 11]. Accordingly, a Electronic supplementary material The online version of this article (https://doi.org/10.1007/s41664-020-00134-z) contains supplementary material, which is available to authorized users. * Dechen Jiang [email protected] 1
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
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robust detection method for simple, sensitive, and specific sensing of hydrogen peroxide is being sought. To date, a variety of methods have been reported for the assay of hydrogen peroxide, such as fluorescence analysis [12, 13], electrochemical detection [14–16], and electrochemiluminescence (ECL) assay [17–19]. Among these methods, ECL analysis has the advantages of high sensitivity, simplified set-up, low background, and g
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