Elevated expression of RUNX3 co-expressing with EZH2 in esophageal cancer patients from India
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Cancer Cell International Open Access
PRIMARY RESEARCH
Elevated expression of RUNX3 co‑expressing with EZH2 in esophageal cancer patients from India Asad Ur Rehman1, Mohammad Askandar Iqbal2, Real Sumayya Abdul Sattar1, Snigdha Saikia3, Mohammad Kashif4, Wasif Mohammad Ali5, Subhash Medhi3, Sundeep Singh Saluja6* and Syed Akhtar Husain1*
Abstract Background: Runt related transcription factor3 (RUNX3) is considered as a tumor suppressor gene (TSG) that functions through the TGF-β dependent apoptosis. Promoter methylation of the CpG islands of RUNX3 and overexpression of enhancer of zeste homolog 2 (EZH2) has been suggested to downregulate RUNX3 in cancer. Methods: Here, we studied the expression of RUNX3 and EZH2 in 58 esophageal tumors along with paired adjacent normal tissue. mRNA levels, protein expressions and cellular localizations of EZH2 and RUNX3 were analyzed using real-time PCR and immunohistochemistry, respectively. DNA methylation was further assessed by the methylation specific-PCR. Results: Compared to normal tissue, a significant increase in expression of RUNX3 mRNA in 31/57 patient’s tumor tissue (p
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