Emodin Inhibits Lipopolysaccharide-Induced Inflammation by Activating Autophagy in RAW 264.7 Cells
- PDF / 858,162 Bytes
- 8 Pages / 612.284 x 824.882 pts Page_size
- 49 Downloads / 199 Views
C
hinese Journal of Integrative Medicine
•1•
Available online at link.springer.com/journal/11655 Journal homepage: www.cjim.cn/zxyjhen/zxyjhen/ch/index.aspx E-mail: [email protected]
Original Article
Emodin Inhibits Lipopolysaccharide-Induced Inflammation by Activating Autophagy in RAW 264.7 Cells TU Yan-jie1,2, TAN Bo3, JIANG Lei1,2, WU Zhong-hua1,2, YU Hong-ji1,2, LI Xiao-qian1,2, and YANG Ai-dong1,2 Objective:: To investigate the effects of emodin on inflammation and autophagy in ABSTRACT Objective Methods:: lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and reveal its underlying mechanism. Methods 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was conducted to find the appropriate dose for emodin. RAW264.7 cells pretreated with different concentrations (0–50 μmol/L) of emodin or vehicle for 2 h prior to exposure to LPS for 16 h. Cell morphology was examined and propidium iodide staining was used to examine cell cycle. Expressions of inflammation-related proteins [nuclear factor-kappaB (NF-κB) and I-kappaB (IκB)α] and autophagy-related proteins [light chain (LC)3, P62/sequestosome 1, mammalian target of rapamycin (mTOR), and p-mTOR] were examined using Western blot analysis. Expression of inflammation-related cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were detected by enzyme-linked immunosorbent assay. Autophagy was examined with LC3B fluorescence intensity and aggregation. The effect of emodin on autophagy was conducted with an Results:: The expression of NF-κB in LPS-induced cells was autophagy inhibitor, 3-methyladenine (3-MA). Results significantly increased (P
Data Loading...
