Encapsulation and Release of Circulating Tumor Cells Using 3D DNA Hydrogels
- PDF / 377,411 Bytes
- 2 Pages / 595.32 x 841.92 pts (A4) Page_size
- 46 Downloads / 206 Views
doi: 10.1007/s40242-020-0251-7
Highlight
Encapsulation and Release of Circulating Tumor Cells Using 3D DNA Hydrogels GUO Zijian* State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China Abstract To minimize the concomitant cell damage in the capture process of circulating tumor cells(CTCs) in conventional methods, Zuo et al. proposed to use aptamer-trigger-clamped hybridization chain reaction method to encapsulate CTCs by in-situ forming 3D DNA hydrogel. The 3D porous DNA hydrogel on cell membrane minimizes cell damage, and the CTCs can be readily released by the ATP triggered dis-assembly of DNA hydrogel for subsequent culture and live cell analysis. This work has been published online in the Nature Protocols in June 22, 2020. How to capture and release circulating tumor cells(CTCs) with minimal cell damage or fragmentation is crucial to maintain the faithful tumor-related molecular information for subsequent downstream analysis. Conventionally, cell damage is difficult to avoid during the capturing process because of the direct collisions between CTCs and the hard microcolumn or the solid interface. To solve this problem, Zuo et al.[1,2] has proposed to use a new soft biomaterial to encapsulate living CTCs. They chose to use DNA molecule, a biocompatible biomolecule to construct soft DNA hydrogel. Using a DNA strand(aptamer of EpCAM is used in this work), which can specifically bind on CTC membrane as an initiator, 3D porous DNA hydrogel can be generated on cell membrane based on a clamped hybridization chain reaction. This porous and soft
Fig.1
DNA hydrogel can maintain the integrity of the cell without changing cell viability. In addition, the optical transparency of DNA hydrogel allows standard staining, labeling, and microscopy imaging for further living cell study. Nondestructive release of the captured CTCs, which is of vital importance for subsequent cell analysis, is always a challenge in the field of CTCs capture. Conventional approaches for CTCs release were mainly relied on fluidic shear force, enzymatic treatment or electrochemical repulsion, all of which showed significant loss for cell viability and molecular integrity. To circumvent this problem, Zuo et al.[1,2] proposed to introduce a small molecular-responsive aptamer in the clamped hybridization chain reaction. Once the small molecule is introduced, it can trigger the disassembly of the 3D DNA networks
DNA hydrogel-based encapsulation and release of CTCs(A), step-loop-structured H1 and H2 in a metastable state, the formation of the 3D networks initiated by initiator and the disassembly of DNA networks triggered by ATP(B), confocal microscopic imaging of the aptamer-initiator biblocks specifically binding on cell membrane(C), FDA dyes-stained cells encapsulated in the 3D DNA hydrogel(D), SEM characterization of a single cell encapsulated by DNA hydrogel(E) and the released cells in solution triggered by the additi
Data Loading...
