Enzymatic Hydrolysis of Agavins to Generate Branched Fructooligosaccharides (a-FOS )
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Enzymatic Hydrolysis of Agavins to Generate Branched Fructooligosaccharides (a-FOS) Alicia Huazano-García 1 & Mercedes G. López 1
Received: 29 March 2017 / Accepted: 26 May 2017 # Springer Science+Business Media New York 2017
Abstract Recently, agavins (branched neo-fructans) of short degree of polymerization have shown beneficial effects on the health of both healthy and overweight individuals. Therefore, the aim of the present work was to investigate the potential use of Agave angustifolia agavins on the generation of branched fructooligosacharides (a-FOS). A. angustifolia agavins were hydrolyzed using exo-, endo-inulinase, and a mixture of both (25 and 75%, respectively). Exoand the inulinase mixture degraded quickly the agavins in relation to endo-inulinase treatment. Only endo-inulinase and the inulinase mixture generated a-FOS formation. Endo-inulinase degraded 31% of agavins, yielding approximately 20% of a-FOS after 48 h, whereas the inulinase mixture hydrolyzed 33% of agavins in just 90 min, but only yielded 10% of a-FOS. These results suggest that agave plants could be an abundant raw material for a-FOS production, which might have a huge prebiotic potential as new branched fructooligosaccharides with many applications in the alimentary and pharmaceutical industry. Keywords Agavins . Branchedfructooligosaccharides(a-FOS) . Agaveangustifolia . Enzymatic hydrolysis
Introduction Mexico is considered the origin and diversification of the Agave genus since a large number of agave species are found in its territory. The Agave genus includes approximately 166 species and is the largest genus among the Agavaceae family that consists of 9 genera and approximately 293 species [1]. Agave tequilana is perhaps the most known commercial species due to it is used for tequila production; however, other Agave species such as A. angustifolia are grown in Mexico for the mezcal production (a distilled beverage similar to tequila) [2].
* Mercedes G. López [email protected]
1
Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apartado Postal 629, Irapuato, 36821 Guanajuato, Mexico
Appl Biochem Biotechnol
The Agave plants present the cassulacean acid metabolism and their principal photosynthetic products are agavins [3]. Agavins are neo-fructans composed of complex and highly branched molecules with β(2–1) and β(2–6) linkages as well as an internal glucose unit [3, 4]. Mancilla-Margalli and López [4] investigated the agavins contents in five Agave species (A. tequilana, A. angustifolia, A. potatorum, A. cantala, and A. fourcroydes) grown in different regions of Mexico. The authors found that A. angustifolia from Oaxaca showed the highest content of agavins (86%) with the largest degree of polymerization (DPaverage = 32) among the studied Agave species. Agavins are very important for the alcoholic beverage industry because they constitute the principal raw material for tequila or mezcal elaboration [5]. However, the industrial interest for agavins
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