Evaluation of adhesion molecules CD64, CD11b and CD62L in neutrophils and monocytes of peripheral blood for early diagno
- PDF / 196,313 Bytes
- 4 Pages / 595.22 x 842 pts (A4) Page_size
- 94 Downloads / 163 Views
Evaluation of adhesion molecules CD64, CD11b and CD62L in neutrophils and monocytes of peripheral blood for early diagnosis of neonatal infection Ferah Genel, Fusun Atlihan, Nesrin Gulez, Elif Kazanci, Canan Vergin, Demet Tumay Terek, Ozlem Cengel Yurdun Izmir, Turkey
Brief report
Background: This study was undertaken to assess the value of neutrophils CD11b, CD64, and CD62L for the early diagnosis of neonatal infection. Methods: Eighty-four neonates who were followed up for a suspected neonatal infection were included in this study. They were assigned into an infection group (n=49) and a non-infection group (n=35). Healthy neonates served as controls (n=35). A full sepsis screening was performed and neutrophil and monocyte expressions of CD11b, CD64 and CD62L were determined by flow cytometry. Results: The expressions of CD64 and CD11b were significantly enhanced in the infection group compared to the non-infective group and the controls. Conclusions: CD64 expression on neutrophils and monocytes is a useful diagnostic marker for the early diagnosis of neonatal infection. Combination of CD64, CD11b and C reactive protein further enhances the sensitivity of the expression and its negative predictive value. World J Pediatr 2012;8(1):72-75 Key words: adhesion molecule; markers; neonatal infection
Author Affiliations: Department of Pediatrics, Dr Behçet Uz Children's Hospital, Alsancak, Izmir, Turkey (Genel F, Atlihan F, Gulez N, Kazanci E, Vergin C, Tumay Terek D, Cengel Yurdun O) Corresponding Author: Ferah Genel, MD, Department of Pediatrics, Dr Behçet Uz Children's Hospital, 1374 sokak, No: 11, 35210, Alsancak, Izmir, Turkey (Tel: +90 232 489 56 56; Fax: +90 232 489 23 15; Email: ferahgen@ yahoo.com) doi: 10.1007/s12519-011-0304-6 ©Children's Hospital, Zhejiang University School of Medicine, China and Springer-Verlag Berlin Heidelberg 2011. All rights reserved.
72
Introduction
T
he clinical signs of neonatal infection are subtle, nonspecific and indistinguishable from those caused by a variety of neonatal noninfectious disorders. Recent advances in flow cytometric technology have provided opportunities for detecting cell surface activation markers on specific cell types in a few hours with a minimal blood volume.[1-6] The present study was undertaken to assess the value of neutrophil adherence molecules CD11b, CD64, and CD62L (L selectin) for the early diagnosis of neonatal infection.
Methods
Neonates Eighty-four neonates who had been treated at the neonatal intensive care unit at Dr. Behcet Uz Children's Hospital in Izmir, Turkey, between March 2006 and April 2007, for a suspected infection were included in this study. Clinical signs and symptoms suggestive of neonatal infection were temperature instability, tachycardia or bradycardia, poor perfusion, shock, apnea, cyanosis, intercostals retractions, tachypnea, hypotonia, lethargy, seizures, abdominal distension, gastrointestinal bleeding or petechiae. Hematological and biochemical laboratory investigations were performed. Leucopenia was defined as leukoc
Data Loading...
