Evaluation of Different Inoculation Methods for Screening of Sclerotinia rot and Phytophthora blight in Perilla Germplas
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J. Crop Sci. Biotech. 2019 (June) 22 (2) : 177 ~ 183 DOI No. 10.1007/s12892-019-0115-0 RESEARCH ARTICLE
Evaluation of Different Inoculation Methods for Screening of Sclerotinia rot and Phytophthora blight in Perilla Germplasm Tania Afroz1, Ho-Sun Lee1*, Young-Ah Jeon2, Jung-Sook Sung1, Ju-Hee Rhee1, Awraris Derbie Assefa1, Jaejong Noh1, Aejin Hwang1, On-Sook Hur1, Na-Young Ro1, Jae-Eun Lee1, Myung-Chul Lee1 1
National Agrobiodiversity Center, National Institute of Agricultural Science, Jeonju, 54874, Rep. of Korea. International Technology Cooperation Center, Rural Development Administration, Jeonju 54875, Rep. of Korea
2
Received: April 23, 2019 / Revised: May 27, 2019 / Accepted: May 29, 2019 Ⓒ Korean Society of Crop Science and Springer 2019
Abstract Sclerotinia rot and Phytophthora blight are devastating diseases of perilla that can cause huge loss of perilla production. Screening disease resistant genetic resources is necessary to develop disease-resistant cultivars and conduct related research. The objective of this study was to find suitable inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. Three different inoculation methods [i.e., detached leaf (agar plug placed on start point of vein, 1/3 point of leaf vein & front side, back side), stem tip, and soil drenching] with three-growth stages (two, four & six-leaf stage) at two different temperatures (20 & 25°C) were assayed for their effectiveness for screening using IT 229019 & Sang-yeop perilla germplasm. Based on disease lesion, 20°C in two-leaf stage with detached leaf method (start point of vein) was found to be an efficient method for screening of Sclerotinia rot whereas 25°C in two-leaf stage with stem tip was an effective method for screening of Phytophthora blight in perilla germplasm. However, stem tip method was laborious as it required more time. Therefore, detached leaf method (start point of vein & back side) was a simple and rapid method that could be preferably used for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. Key words : Detached leaf, Perilla frutescens, Phytophthora blight, Sclerotinia rot, stem tip
Introduction Perilla (Perilla frutescens L.) is an upright, bushy annual herbaceous plant that belongs to mint family Limaceae. Perilla is native from the Himalayas to Southeast Asia. It is now widely distributed in many regions of the world (Lee and Ohnishi 2003). Since perilla has different bioactive materials, it has been used as an important traditional herbal medicine for treating various diseases, including depression, anxiety, tumor, cough, antioxidant, allergy, intoxication, and some intestinal disorders (Ueda et al. 2002; Ichiishi and Yoshikawa 2004; Lin et al. 2010; Saklani et al. 2011). In Asian countries such as Japan, Korea, and China, its young leaves are commonly added to sushi, garnishes, and soups or used to wrap cooked food. Its older leaves can also be used as a garnish or flavoring. Its leaves can also be dried for later use. Moreover, per
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