Fed-batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arab
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RESEARCH PAPER
Fed‑batch polyhydroxybutyrate production by Paraburkholderia sacchari from a ternary mixture of glucose, xylose and arabinose Mengxing Li1,2 · Mark R. Wilkins1,3,4,5 Received: 6 January 2020 / Accepted: 24 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Polyhydroxybutyrate (PHB) is a biodegradable bioplastic that is comparable with many petroleum-based plastics in terms of mechanical properties and is highly biocompatible. Lignocellulosic biomass conversion into PHB can increase profit and add sustainability. Glucose, xylose and arabinose are the main monomer sugars derived from upstream lignocellulosic biomass processing. The sugar mixture ratios may vary greatly depending on the pretreatment and enzymatic hydrolysis conditions. Paraburkholderia sacchari DSM 17165 is a bacterium strain that can convert all three sugars into PHB. In this study, fed-batch mode was applied to produce PHB on three sugar mixtures (glucose:xylose:arabinose = 4:2:1, 2:2:1, 1:2:1). The highest PHB concentration produced was 67 g/L for 4:2:1 mixture at 41 h corresponding to an accumulation of 77% of cell dry weight as PHB. Corresponding sugar conversion efficiency and productivity were 0.33 g PHB/g sugar consumed and 1.6 g/L/h, respectively. The results provide references for process control to maximize PHB production from real sugar streams derived from corn fibre. Keywords Lignocellulosic biomass · Ternary sugar mixture · Glucose, xylose and arabinose · Fed-batch · Polyhydroxybutyrate
Introduction Lignocellulosic biorefinery can produce multiple valuable bioproducts (ethanol, butanol, succinic acid, lipids, enzymes and polyhydroxybutyrate) [1–7]. These bioproducts have the potential to add profit and promote sustainability in the agricultural industry and rural economy. The process development associated with these products are widely studied using various lignocellulosic feedstocks for the past decade [7–13]. Of these bioproducts, polyhydroxybutyrate * Mark R. Wilkins [email protected] 1
Department of Biological Systems Engineering, The University of Nebraska-Lincoln, Lincoln 68583, NE, USA
2
Department of Statistics, The University of Nebraska-Lincoln, Lincoln 68583, NE, USA
3
Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln 68588, NE, USA
4
Industrial Agricultural Products Center, University of Nebraska-Lincoln, Lincoln 68583, NE, USA
5
Present Address: 211 Chase Hall, 3605 Fair St, Lincoln 68583, NE, USA
(PHB) is a polymer of particular interest due to its comparable mechanical properties to many petroleum-based plastics and its high biocompatibility [14]. Commercial PHB produced by microbes is still 3 times more expensive than petroleum-based plastics [15–17]. Raw materials make up about 50% of the production cost of PHB [18–20]. Lignocellulosic biomass conversion into PHB could increase the value of lignocellulosic feedstocks, decrease costs of PHB production and promote sustainability of corn biorefineries. After upstream pro
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