Fine mapping of a candidate gene for cool-temperature-induced albinism in ornamental kale
- PDF / 7,453,107 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 44 Downloads / 202 Views
RESEARCH ARTICLE
Open Access
Fine mapping of a candidate gene for cooltemperature-induced albinism in ornamental kale Chenghuan Yan1,2, Liying Peng1, Lei Zhang1 and Zhengming Qiu2*
Abstract Background: The symptoms of cool-temperature-induced chlorosis (CTIC) are widely existed in higher plants. Although many studies have shown that the genetic mechanism of CTIC is generally controlled by recessive genes in model plants, the dominant inheritance of albinism has not been reported thus far. Here, two CTIC mutants, Red Kamome and White Kamome, were utilized to analyse the inheritance of the albino trait in ornamental kale. The objective of this investigation is to fine-map the target locus and identify the most likely candidate genes for albinism. Results: Genetic analysis revealed that the albinism in the inner leaves of ornamental kale followed semi-dominant inheritance and was controlled by a single locus in two segregating populations. BSR-seq in combination with linkage analysis was employed to fine-map the causal gene, named AK (Albino Kale), to an approximate 60 kb interval on chromosome C03. Transcriptome data from two extreme pools indicated that the differentially expressed gene of Bol015404, which encodes a cytochrome P450 protein, was the candidate gene. The Bol015404 gene was demonstrated to be upregulated in the albino leaves of ornamental kale by qPCR. Additionally, the critical temperature for the albinism was determined between 10 °C and 16 °C by gradient test. Conclusions: Using two independent segregating populations, the albino mutants were shown to be controlled by one semi-dominant gene, AK, in ornamental kale. The Bol015404 gene was co-segregated with albinism phenotypes, suggesting this unknown function P450 gene as the most likely candidate gene. The albino trait appeared caused by the low temperatures rather than photoperiod. Our results lay a solid foundation on the genetic control of albinism in ornamental kale. Keywords: Albino trait, Semi-dominant inheritance, BSR-seq, Cytochrome P450 gene, Ornamental kale
Background Chlorophyll biosynthesis is the most important biochemical process on our planet [1]. The chlorophyll biosynthetic pathway occurs in chloroplasts, and involves many enzyme-catalysed reactions [2]. Therefore, chloroplasts are unique units of photosynthesis in green plants that generate multiple metabolic products of the * Correspondence: [email protected] 2 Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, People’s Republic of China Full list of author information is available at the end of the article
photosynthetic processes [3]. Numerous studies have reported that chloroplasts are derived from proplastids, and the formation of chloroplasts involves the regulation of plastid and nuclear genes [4]. To date, substantial evidences have revealed that the biogenesis of chloroplasts is precisely regulated by a series of genes. Low temperature is a common abiotic
Data Loading...
