Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian
Gastruloids are embryonic organoids made from small, defined numbers of mouse embryonic stem cells (mESCs) aggregated in suspension culture, which over time form 3D structures that mimic many of the features of early mammalian development. Unlike embryoid
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Mo R. Ebrahimkhani Joshua Hislop Editors
Programmed Morphogenesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.
Programmed Morphogenesis Methods and Protocols
Edited by
Mo R. Ebrahimkhani Department of Pathology, Division of Experimental Pathology,School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA
Joshua Hislop Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA, USA; Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pathology, Division of Experimental Pathology,School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
Editors Mo R. Ebrahimkhani Department of Pathology Division of Experimental Pathology School of Medicine University of Pittsburgh Pittsburgh, PA, USA Pittsburgh Liver Research Center University of Pittsburgh Pittsburgh, PA, USA Department of Bioengineering Swanson School of Engineering University of Pittsburgh Pittsburgh, PA, USA
Joshua Hislop Department of Bioengineering Swanson School of Engineering University of Pittsburgh Pittsburgh, PA, USA Pittsburgh Liver Research Center University of Pittsburgh Pittsburgh, PA, USA Department of Pathology Division of Experimental Pathology School of Medicine University of Pittsburgh Pittsburgh, PA, USA
McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-1173-9 ISBN 978-1-0716-1174-6 (eBook) https://doi.org/10.1007/978-1-0716-1174-6 © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media,
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