Gene profiling, biomarkers and pathways characterizing HCV-related hepatocellular carcinoma
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Gene profiling, biomarkers and pathways characterizing HCV-related hepatocellular carcinoma Valeria De Giorgi1,2, Alessandro Monaco3, Andrea Worchech3,4,5, MariaLina Tornesello1, Francesco Izzo6, Luigi Buonaguro1, Francesco M Marincola3, Ena Wang3 and Franco M Buonaguro*1 Address: 1Molecular Biology and Viral Oncogenesis & AIDS Refer. Center, Ist. Naz. Tumori "Fond. G. Pascale", Naples - Italy, 2Department of Chemistry, University of Naples "Federico II", Naples, Italy, 3Infectious Disease and Immunogenetics Section (IDIS), Department of Transfusion Medicine, Clinical Center and Trans-NIH Center for Human Immunology (CHI), National Institutes of Health, Bethesda, MD -USA, 4Genelux Corporation, Research and Development, San Diego Science Center, San Diego, CA, USA, 5Department of Biochemistry, Biocenter, University of Wuerzburg, Am Hubland, Wuerzburg, Germany and 6Div. of Surgery "D", Ist. Naz. Tumori "Fond. G. Pascale", Naples - Italy Email: Valeria De Giorgi - [email protected]; Alessandro Monaco - [email protected]; Andrea Worchech - [email protected]; MariaLina Tornesello - [email protected]; Francesco Izzo - [email protected]; Luigi Buonaguro - [email protected]; Francesco M Marincola - [email protected]; Ena Wang - [email protected]; Franco M Buonaguro* - [email protected] * Corresponding author
Published: 12 October 2009 Journal of Translational Medicine 2009, 7:85
doi:10.1186/1479-5876-7-85
Received: 2 July 2009 Accepted: 12 October 2009
This article is available from: http://www.translational-medicine.com/content/7/1/85 © 2009 De Giorgi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Hepatitis C virus (HCV) infection is a major cause of hepatocellular carcinoma (HCC) worldwide. The molecular mechanisms of HCV-induced hepatocarcinogenesis are not yet fully elucidated. Besides indirect effects as tissue inflammation and regeneration, a more direct oncogenic activity of HCV can be postulated leading to an altered expression of cellular genes by early HCV viral proteins. In the present study, a comparison of gene expression patterns has been performed by microarray analysis on liver biopsies from HCV-positive HCC patients and HCV-negative controls. Methods: Gene expression profiling of liver tissues has been performed using a high-density microarray containing 36'000 oligos, representing 90% of the human genes. Samples were obtained from 14 patients affected by HCV-related HCC and 7 HCV-negative non-liver-cancer patients, enrolled at INT in Naples. Transcriptional profiles identified in liver biopsies from HCC nodules and paired non-adjacent non-HCC liver tissue of the same HCV-positive patients were compared to those from HCV-negative controls by the Cluster progra
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