Green synthesis of salt-tolerant gold nanoparticles for the rapid qualitative detection of Listeria monocytogenes in lat
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Green synthesis of salt-tolerant gold nanoparticles for the rapid qualitative detection of Listeria monocytogenes in lateral flow immunoassay Juan Du1,2,3, Shujing Wu1, Zheyuan Hu4, Ziyue Yu1, Dianbo Zhao1,2,3, and Yanhong Bai1,2,3,*
1
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Room 207, No.136 Kexue road, Zhengzhou 450001, Henan Province, China 2 Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China 3 Henan Collaborative Innovation Center of Food Production and Safety, Zhengzhou 450001, China 4 College of Electrical and Information Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China
Received: 9 March 2020
ABSTRACT
Accepted: 17 August 2020
Gold nanoparticles (AuNPs)-based lateral flow immunoassay (LFIA) is a widely used detection technique. Here, we developed a novel method for green synthesis of a salt-tolerant AuNPs by using aqueous extract of Damask rose petals (AEDR), for the application of Listeria monocytogenes (L. monocytogenes) detection by LFIA. Key parameters were optimized to achieve an improved optical LFIA performance, and the detection limit and specificity were further studied. AEDR-AuNPs were synthesized within 1 min at RT by AEDR at a concentration of 240 lg/mL, and the method was rapid, simple, economical and environmentally friendly. The AEDR-AuNPs were spherical with the size mainly ranging from 20 to 28 nm, and the salt stability was determined to be 0.4 M NaCl, which is 10 times higher than AuNPs synthesized by citrate reducing method. The optimized immunization concentration of anti-Listeria monocytogenes polyclonal antibody to AEDR-AuNPs was 60 lg/mL. The optimized antibody concentrations of test (T) line and control (C) line were determined as 1 mg/mL and 0.5 mg/mL, respectively. The detection limit was 2.5 9 105 CFU/mL and 2.85 9 105 CFU/mL in pure L. monocytogenes culture and pork tenderloin sample, respectively, and the result can be read by naked eye within 10 min. The antibody–AEDR-AuNPs LFIA strip showed no cross-reaction with all the tested bacteria including Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Bacillus subtilis, Escherichia coli O157:H7 and Salmonella typhimurium. This approach showed a promising application for the detection of L. monocytogenes concerning food safety.
Published online: 25 August 2020
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Springer Science+Business
Media, LLC, part of Springer Nature 2020
Handling Editor: Yaroslava Yingling.
Address correspondence to E-mail: [email protected]
https://doi.org/10.1007/s10853-020-05118-z
J Mater Sci (2020) 55:15426–15438
Introduction The gram-positive bacterium Listeria monocytogenes (L. monocytogenes) was first isolated and characterized from a septicemic infection of rabbits in 1928, by EGD Murray et al. in England [1]. L. monocytogenes is a ubiquitous foodborne pathogen that has become an important cause of human foodborne infections worldwide [2, 3]. Contamination of ready-to-eat food by L. monocytogenes is thoug
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