High frequency direct plant regeneration, micropropagation and Shikonin induction in Arnebia hispidissima
- PDF / 467,462 Bytes
- 7 Pages / 609.451 x 793.701 pts Page_size
- 35 Downloads / 171 Views
J. Crop Sci. Biotech. 2010 (March) 13 (1) : 13 ~ 20 DOI No. 10.1007/s12892-009-0127-3 RESEARCH ARTICLE
High Frequency Direct Plant Regeneration, Micropropagation and Shikonin Induction in Arnebia hispidissima Minakshi Pal, Ashok Chaudhury* Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001 (Haryana), India Received: September 22, 2009 / Accepted: November 26, 2009 Ⓒ Korean Society of Crop Science and Springer 2010
Abstract The data presented herein reports a rapid and efficient method for direct plant regeneration at high frequency without intervening callus formation from shoot tip (93%) and nodal segment (60%) cultured on MS media supplemented with 0.5 mg l-1 KIN, 0.25 mg l-1 BAP, 0.1 mg l-1 IAA and 100 mg l-1 CH. Conversely, leaf and internodal explants were poorly responsive. Adventitious shoot buds arose not only from the cut ends but all along the surface of the explants leading to the formation of clusters with multiple shoots. Multiple shoots upon transfer to MS media supplemented with 2.0 mg l-1 IBA induced efficient rooting (80%). In vitro flowering was observed when tissue culture-raised plantlets were maintained for extended period in culture. Shikonin was induced in roots of regenerated plants which often exudates in the culture medium was quantified spectrophotometerically by recording absorbance at 620 nm and estimated to be 0.50 mg g-1 fresh weight of tissue at the end of the 50 days of culture. The regenerated plants were successfully acclimatized, hardened, and transferred to soil in green house for micropropagation. The protocol developed here will be very useful for the supply of Arnebia hispidissima all year as a raw product necessary for obtaining Shikonin for the cosmetic, dyeing, food, and pharmaceutical industries. Key words: Arnebia hispidissima, callus, direct plant regeneration, in vitro, shikonin Abbreviations: BAP-6-benzylaminopurine; CH-Casein hydrolysate; IAA-Indole acetic acid; IBA-Indole butyric acid; KIN-Kinetin; NAA-naphthalene acetic acid.
Introduction The genus Arnebia belongs to the family Boraginaceae comprising nearly twenty-five different species which includes a variety of herbs, shrubs, and trees, mostly confined to Asia with a few species occurring in the drier parts of North Africa. Seven species are known to occur in India which includes A. benthami, A. euchroma, A. guttata, A. hispidissima, and A. nobilis. Plants of the genus Arnebia as well as some other species of Boraginaceae belonging to genera Echium, Lithospermum, and Onosma are the source of naphthoquinone-Shikonin known since ancient times as a red-colored dye used for the silk and food industries. Minakshi Pal, Ashok Chaudhury ( ) E-mail: [email protected] Phone: +91-1662-263306 Fax: +91-1662-276240
The Korean Society of Crop Science
Shikonin is known for its tremendous application in the food, health care, and pharmaceuticals industries and possesses antibacterial and antifungal activities and exhibits anti-inflammatory and wound-healing prope
Data Loading...
