IAA-producing Klebsiella variicola AY13 reprograms soybean growth during flooding stress
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J. Crop Sci. Biotech. 2017 (December) 20 (4) : 235 ~ 242 DOI No. 10.1007/s12892-017-0041-0 RESEARCH ARTICLE
IAA-producing Klebsiella variicola AY13 Reprograms Soybean Growth During Flooding Stress Ah-Young Kim1, Raheem Shahzad1, Sang-Mo Kang1, Chang-Woo Seo1, Yeon-Gyeong Park1, Hyun-Jin Park2, In-Jung Lee1* 1
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea
2
Received: May 19, 2017 / Revised: September 02, 2017 / Accepted: November 11, 2017 Ⓒ Korean Society of Crop Science and Springer 2017
Abstract Soybean is known to be intolerant of waterlogged conditions. Flooding stress usually affects the crop via poor and retorted root development. The current study aimed to isolate indole acetic acid (IAA)-producing bacteria with potential to induce adventitious root growth and counteract flooding stress. The isolate AY-13 was identified as Klebsiella variicola using 16S DNA sequencing and phylogenetic analysis. A pure culture of AY-13 Klebsiella variicola was subjected to chromatography and mass spectrometry selected-ion monitoring (GC-MS/SIM) for IAA quantification. The results revealed the presence of (84.27±3.55 µg/mL) IAA in the AY-13 culture. The strain AY-13 was able to induce adventitious root initiation. Therefore, soybean seedlings were inoculated with AY-13 to examine its potential for promoting growth and reprograming after flooding stress. AY-13 application not only mitigated the flooding stress, but also significantly improved the plants’ growth, enhanced chlorophyll contents, and improved the quantum efficiency of chlorophyll fluorescence during and after flooding stress. Our findings were that AY-13 possesses great potential for mitigating flooding stress and improving soybean plant growth. Key words : Klebsiella variicola, IAA, flooding stress, soybean
Introduction Plants are surrounded by diverse environments and are frequently subjected to environmental changes that cause various abiotic stresses such as high and low temperature, drought, flooding, and salinity (Tester and Bacic 2015). These abiotic stresses are all involved in restricting agricultural productivity through effects on plant growth and development on physiological, morphological, and molecular levels (Gray and Brady 2016). In response to these abiotic stresses, plants adopt different physiological and metabolic modulations that enhance tolerance. In addition, certain soil microbes encourage plant growth and can help the plants avoid or overcome a variety of environmental stresses (Zhu 2016; Figueiredo et al. 2010). Plant growth-promoting bacteria facilitate plant growth and improve tolerance against various environmental stress by both direct and indirect mechanisms (Zhou et al. 2016; In-Jung Lee () Email: [email protected] Tel: +82539505708 Fax: +82539536972
The Korean Society of Crop Science
Kang et al. 2015). Direct mechanisms involve nitrogen fixation and phosphate solubilization, fac
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