Identification of Dysfunctional Gut Microbiota Through Rectal Swab in Patients with Different Severity of Acute Pancreat
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ORIGINAL ARTICLE
Identification of Dysfunctional Gut Microbiota Through Rectal Swab in Patients with Different Severity of Acute Pancreatitis Shanshan Yu1 · Yangyang Xiong2 · Jun Xu1 · Xianquan Liang3 · Yangyang Fu1 · Danyu Liu1 · Xuezhong Yu1 · Dong Wu2 Received: 28 September 2019 / Accepted: 9 January 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background Acute pancreatitis (AP) has a wide spectrum of severity and can be associated with considerable morbidity and mortality. Whether gut microbiota dysbiosis is associated with AP severity remains obscure. Aims We aim to investigate the differences in the alterations of gut microbiota in different grades of AP severity. Methods We collected clinical information and rectal swab samples from 80 individuals. The gut microbiota was tested by 16S rRNA gene sequencing, gut microbiota species composition analysis, difference analysis, random forest model prediction analysis, and gut microbiota species correlation network analysis. Results There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant species in mild, moderately severe, and severe AP, respectively. Finegoldia was the most significantly increased and Blautia the most decreased species in mild AP. Anaerococcus was the most significantly increased and Eubacterium hallii the most decreased species in moderately severe AP. Enterococcus was the most significantly increased and Eubacterium hallii the most decreased species in severe AP. Finegoldia, Eubacterium_hallii, and Lachnospiraceae were potential diagnostic biomarkers for mild AP and Eubacterium_hallii and Anaerococcus for moderately severe AP. There was a positive interaction between Firmicutes and Bacteroidetes in mild AP. Conclusions The disturbed gut microbiota is different among grades of AP, suggesting their potential role in the progression of disease severity. Graphic Abstract There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant gut microbiota species in MAP, MSAP, and SAP, respectively. Finegoldia was the most significantly increased Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10620-020-06061-4) contains supplementary material, which is available to authorized users. * Dong Wu [email protected]
Xuezhong Yu [email protected]
Shanshan Yu [email protected]
1
Department of Emergency Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng District, Beijing 100730, China
2
Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng District, Beijing 100730, China
3
Department of Emergency Medicine, The Second People’s Hospital of Guiyang, Guiyang 550004, China
Yangyang Xiong xiong_yangyan
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