Identification of loci for pre-harvest sprouting resistance in the highly dormant spring wheat RL4137
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ORIGINAL ARTICLE
Identification of loci for pre‑harvest sprouting resistance in the highly dormant spring wheat RL4137 M. M. Uzzal A. Liton1 · Curt A. McCartney2 · Colin W. Hiebert2 · Santosh Kumar3 · Mark C. Jordan2 · Belay T. Ayele1 Received: 23 May 2020 / Accepted: 11 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Combination of RL4137 alleles at three QTLs on chromosomes 4A, 6B and 6D, and ‘Roblin’ allele at a novel QTL on chromosome 1D increases pre-harvest sprouting resistance in ‘Roblin’/RL4137 doubled haploid population. Abstract Pre-harvest sprouting (PHS) significantly reduces wheat grain yield and quality. Therefore, identifying quantitative trait loci (QTL) for PHS resistance is key to facilitate marker-assisted breeding. To this end, we studied PHS in a population of 330 doubled haploid (DH) lines derived from ‘Roblin’/RL4137. The parental and DH lines were examined for their PHS phenotype based on speed of germination index in five environments and genotyped using the wheat Infinium 90 K SNP array. A total of five QTLs were detected on linkage groups 1D, 4A.2, 6B.1, 6D and 7A over the five environments. The QTL QPhs.umb-4A on linkage group 4A.2 was the most consistent across all environments and explained 40–50% of phenotypic variation. The QTL on 1D is a novel QTL and explained 1.99–2.33% of phenotypic variation. The QTLs on 6B.1 and 6D each explained 3.09–4.33% and 1.62–2.45% of phenotypic variation, respectively. A combination of four stable QTLs on linkage groups 1D, 4A.2, 6B.1 and 6D greatly increased PHS resistance. Allelic effects for the QTLs QPhs.umb-4A, QPhs. umb-6B and QPhs.umb-6D were contributed by RL4137, whereas ‘Roblin’ contributed the resistant allele for QPhs.umb1D. QPhs.umb-4A was required for strong dormancy in the ‘Roblin’/RL4137 DH population, and the presence of QTLs QPhs.umb-1D, QPhs.umb-6B and QPhs.umb-6D incrementally increased dormancy; DH lines carrying all four QTLs are considerably more dormant than those carrying only QPhs.umb-4A or none of the four QTLs. Thus, the QTLs identified in this study have the potential to improve PHS resistance in spring wheat.
Introduction Hexaploid wheat (Triticum aestivum L.) is the second most produced cereal crop worldwide. Its economic importance is reflected by the fact that over 40% of the world population Communicated by Susanne Dreisigacker. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03685-y) contains supplementary material, which is available to authorized users. * Belay T. Ayele [email protected] 1
Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T 2N2, Canada
2
Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB R6M 1Y5, Canada
3
Brandon Research and Development Centre, Agriculture and Agri-Food Canada, Brandon, MB R7A 5Y3, Canada
uses it as their staple food, contributing approximately 20% of the total calories
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