In vitro bioactivity and osteoblast cell viability studies of hydroxyapatite-incorporated silica aerogel
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ORIGINAL PAPER: SOL-GEL AND HYBRID MATERIALS FOR BIOLOGICAL AND HEALTH (MEDICAL) APPLICATIONS
In vitro bioactivity and osteoblast cell viability studies of hydroxyapatite-incorporated silica aerogel Nor Suriani Sani1 Nik Ahmad Nizam Nik Malek Halimaton Hamdan5 ●
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Khairunadwa Jemon2 Mohammed Rafiq Abdul Kadir4 ●
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Received: 26 May 2020 / Accepted: 30 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Bioactivity and osteoblastic growth of hydroxyapatite (HA)-incorporated silica aerogels (SA) (HAESA) with different ratio of HA/SiO2 (0.1, 0.5, 1.0, and 1.3 HA/SiO2) were investigated. Bioactivity of HAESA was studied by immersing all samples in simulated body fluids (SBF) for 7 and 14 days. The recovered samples were then characterised using Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy combined with energy dispersive X-ray analyser (FESEM–EDX) and the analysis of phosphate loss in the solution. These samples were also investigated for their effect on cell viability and proliferation abilities against normal human osteoblast cells in vitro after 24 and 48 h of exposure times. The HA/SiO2 ratio affected its bioactivity in which the bioactivity increased to 0.5 (HAESA-0.5) and declined at higher ratios (1.0 and 1.3). A similar trend was observed for cell viability and proliferation assays. HAESA-0.5 (HA/SiO2 ratio of 0.5), which possesses the optimal characteristics of SA and HA, resulted in the highest osteoblastic growth due to the synergistic effects between SA and HA. Thus, HAESA-0.5 could be an alternative biomaterial compared to silicaand HA-based biomaterials for bone implant application due to its higher bioactivity in SBF with the improved performance of osteoblast growth. Graphical Abstract Hydroxyapatite-incorporated silica aerogel with different weight ratio of HA/SiO2 (HAESA) was studied for their bioactivity in simulated body fluid and in vitro osteoblastic growth.
* Nik Ahmad Nizam Nik Malek [email protected] 1
Office of Deputy Vice Chancellor (Research and Innovation), Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
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Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
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Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina
Institute for Scientific and Industrial Research (ISI-SIR), Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia 4
Medical Devices and Technology Group (MEDITEG), Faculty of Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
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Razak School of Engineering & Advanced Technology, Universiti Teknologi Malaysia, 54100 Kuala Lumpur, Malaysia
Journal of Sol-Gel Science and Technology
Keywords
Silica aerogel Hydroxyapatite Biomaterial Biocompatibility Osteoblast cells Rice husk ash ●
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Highlights Hydroxyapatite-incorporated silica aerogels (HAESA) has higher bioactivity than silica aerogel. ● HAESA has higher bioactivity than free hydrox
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