Influence of thermodisinfection on microstructure of human femoral heads: duration of heat exposition and compressive st
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Influence of thermodisinfection on microstructure of human femoral heads: duration of heat exposition and compressive strength Christian Fo¨lsch . Julian Dharma . Carlos Alfonso Fonseca Ulloa . Katrin Susanne Lips . Markus Rickert . Axel Pruss . Alexander Jahnke
Received: 13 January 2020 / Accepted: 30 March 2020 Ó The Author(s) 2020
Abstract Allogeneic bone derived from living donors being necessary to match demand for bone transplantation and thermodisinfection of femoral heads is an established sterilization method. During the thermodisinfection the peripheral bone is exposed to maximum 86 °C for 94 min providing 82.5 °C within the center of the femoral head for at least 15 min. This study examined the compression force of the central and representative peripheral regions of native and thermodisinfected human femoral heads to observe wether different duration and intensity of heat exposure might alter mechanic behaviour. Slices from the equatorial region of human femoral heads were taken from each 14 native and thermodisinfected C. Fo¨lsch (&) M. Rickert Department of Orthopaedic Surgery, Justus-LiebigUniversity Medical School, Klinikstrasse 33, 35392 Giessen, Germany e-mail: [email protected] J. Dharma C. A. Fonseca Ulloa A. Jahnke Labarotory of Biomechanics, Department of Orthopaedic Surgery, Justus-Liebig-University Medical School, Klinikstrasse 29, 35392 Giessen, Germany K. S. Lips Laboratory of Experimental Trauma Surgery, JustusLiebig-University, Aulweg 128, 35392 Giessen, Germany
human femoral heads. The central area revealed a significantly higher compression force for native (p B 0.001) and for thermodisinfected bone (p = 0.002 and p = 0.005) compared with peripheral regions since no relevant differences were found between the peripheral and intermediate areas themselves. A small reduction of compression force for thermodisinfected bone was shown since this did not appear significant due to the small number of specimens. The heat exposure did not alter the pre-existing anatomical changes of the microarchitecture of the native femoral heads from the center towards the peripheral regions. The heterogeneity of microstructure of the femoral head might be of interest concerning clinical applications of bone grafts since the difference between native and thermodisinfected bone appears moderate as shown previously. The different quantity of heat exposure did not reveal any significant influence on compression force which might enable thermodisinfection of preformed bone pieces for surgical indications. Keywords Allogeneic bone Human femoral head Compression force Microarchitecture bone Thermodisinfection Bone bank procedures
A. Pruss University Tissue Bank, Institute of Transfusion Medicine, Charite´ University Medical School, Charite´platz 1, 10117 Berlin, Germany
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Cell Tissue Bank
Introduction Allogeneic bone is necessary to match the growing demand for bone replacement material in orthopaedi
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