Infradian Rhythm in Proliferative Activity of a Culture of Embryonic Fibroblast-Like Cells from C57BL/6 Mice
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 5, September, 2020 MORPHOLOGY AND PATHOMORPHOLOGY
Infradian Rhythm in Proliferative Activity of a Culture of Embryonic Fibroblast-Like Cells from C57BL/6 Mice M. A. Diatroptova1, E. A. Ponomarenko1, and M. E. Diatroptov2
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 648-652, May, 2020 Original article submitted February 27, 2020 We studied daily dynamics of proliferative activity of embryonic fibroblast-like cells in culture during the logarithmic growth phase. Daily increase in cell count in the culture showed a 4-day rhythm that persisted over 3 weeks of culturing. In cultures from different animals, the phase of this rhythm was synchronous and did not depend on the moment of cell isolation. It can be hypothesized that the 4-day biorhythm of proliferative activity of embryonic fibroblast-like cells in culture is determined by external environmental factors, probably of electromagnetic nature. Key Words: infradian rhythm; proliferative activity; fibroblast-like cells; cell culture Infradian (multi-day) rhythms have been found in the dynamics of various endocrine and immune parameters. In particular, near 4-day rhythm is present in the dynamics of the concentration of glucocorticoid hormones, testosterone, proliferative activity of the epithelium, morphofunctional parameters of the thymus and spleen, blood concentration of cytokines, and motor activity [4,10-13]. Synchronizers and mechanisms underlying the formation of infradian biorhythms have not been established yet. Based on published data, it can be assumed that quasi-rhythmic oscillations of the electromagnetic field of the Earth can be a synchronizer of infradian rhythms [6,9], because the pineal gland has sensitivity to that factor [14,15]. Earlier, we established a 4- and 12-day rhythm of the mitotic index changes in the esophagus epithelium of male Wistar rats [3]. The antiphase of 4-day rhythms of the mitotic index and the concentration of corticosterone that suppresses proliferative activity of the epithelium was revealed. The 12-day rhythm of mitotic activity is probably mediated by the interaction of the 4-day rhythm of glucocorticoid hormones inResearch Institute of Human Morphology; 2A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia. Address for correspondence: [email protected]. M. E. Diatroptov 1
hibiting proliferation and the 3-day rhythm of thyroid hormones stimulating cell proliferation [2]. Thus, the proliferative activity of cells in the body is determined by a complex of hormonal factors that also demonstrate infradian rhythm. To exclude the effect of internal humoral factors of the body on proliferative activity of cells, it is advisable to study the mechanisms of formation of infradian biorhythms in cell culture. We found no published data about biorhythms of proliferative activity of cultured cells in the infradian range. The aim of this work was to study the dynamics of proliferative
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