Interplay between hydrogen sulfide and methylglyoxal initiates thermotolerance in maize seedlings by modulating reactive
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ORIGINAL ARTICLE
Interplay between hydrogen sulfide and methylglyoxal initiates thermotolerance in maize seedlings by modulating reactive oxidative species and osmolyte metabolism Xin-Yu Ye 1,2,3 & Xue-Mei Qiu 1,2,3 & Yu-Ying Sun 1,2,3 & Zhong-Guang Li 1,2,3 Received: 19 February 2020 / Accepted: 23 May 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Hydrogen sulfide (H2S) and methylglyoxal (MG) were supposed to be novel signaling molecules in plants. However, whether interplay between H2S and MG can initiate thermotolerance in maize seedlings and in relation to metabolism of reactive oxygen species (ROS) and osmolytes is little known. In this study, watering with MG and NaHS (H2S donor) alone or in combination elevated survival and tissue vigor of maize seedlings under heat stress and coped with an increase in the biomembrane injury (as indicated in membrane lipid peroxidation and electrolyte leakage). The above-mentioned effects were separately weakened by MG scavengers (N-acetyl cysteine: NAC; aminoguanidine: AG) and H2S inhibitor (DL-propargylglycine, PAG) and scavenger (hypotaurine, HT). These suggested that the interplay between H2S and MG initiated the thermotolerance in maize seedlings. The further data indicated that, under non-heat stress and heat stress conditions, MG and NaHS alone or in combination modulated ROS metabolism by regulating the activities of antioxidant enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase) and the contents of non-enzymatic antioxidants (ascorbic acid, glutathione, flavonoids, and carotenoids) in maize seedlings. In addition, MG and NaHS alone or in combination also separately modulated the metabolism of osmolytes (proline, trehalose, glycine betaine, and total soluble sugar), H2S (L-cysteine desulfhydrase and O-acetylserine (thione) lyase), and MG (glyoxalase I, glyoxalase II, and MG reductase). These physiological effects also were separately impaired by NAC, AG, PAG, and HT. The current data illustrated that the interplay between H2S and MG initiated the thermotolerance in maize seedlings by modulating ROS, osmolyte, H2S, and MG metabolism. Keywords Hydrogen sulfide . Maize seedlings . Methylglyoxal . Osmolyte . Reactive oxygen species . Signaling interaction . Thermotolerance
Introduction Xin-Yu Ye and Xue-Mei Qiu contributed equally to this work. Handling Editor: Néstor Carrillo * Zhong-Guang Li [email protected] 1
School of Life Sciences, Yunnan Normal University, Kunming 650092, People’s Republic of China
2
Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650092, People’s Republic of China
3
Key Laboratory of Biomass Energy and Environmental Biotechnology, Yunnan Normal University, Kunming 650092, Yunnan Province, People’s Republic of China
Hydrogen sulfide (H2S) is an intermediate product of sulfur in plants, which is a precursor of cysteine synthesizing sulfurco
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