Investigation into the strain typing of Potato Virus Y isolates in Egypt
- PDF / 1,010,491 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 114 Downloads / 229 Views
ORIGINAL ARTICLE
Investigation into the strain typing of Potato Virus Y isolates in Egypt Esraa A. Elwan1 · Mona Rabie1 · Engy E. Abdel Aleem1 · Radwa M. Taha2 · Faiza A. Fattouh1 Received: 3 June 2020 / Accepted: 26 October 2020 © Deutsche Phytomedizinische Gesellschaft 2020
Abstract Potato Virus Y (PVY) is well recognized world-wide as an important virus of potato with damaging effect on both the crop yield and quality. Recent research has revealed a wide range of PVY strain diversity and complexity. The high degree of PVY recombinant types reported in some areas, has intrigued investigation into the efficacy of tools used for strain identification. PCR and ELISA are routinely and reliably used for PVY detection, however, the degree of information reflecting strain genotypes by various identification tools is variable and incoherent. Overall incidence of PVY from different potato samples collected in 2018/2019 in some locations in Egypt is estimated by RT-PCR to be 12%. The most prevalent strain detected was of PVYN origin. Phylogeny analysis revealed high degree of relatedness to isolates from specific locations, which may indicate probable origin of the isolates. Identification of recombinant strains of PVYN origin by both M-RT-PCR and ELISA has indicated that PVYNTN, PVYN−Wilga and PVYN:O are the most prevalent recombinant types. Keywords Potato · PVY · Egypt · Strain genotype
Introduction Potato virus Y (PVY) is regarded as the most common and the most important virus affecting potato (Solanum tuberosum L.) world-wide (Kumar et al. 2014). PVY belongs to the Genus Potyvirus of the Family Potyviridae. The viral genome is a single-stranded, positive-sense RNA of approximately 9.7 kb. As with all potyviruses, there is a poly (A) tail at the 3′terminus and a covalently linked VPg protein at the 5′terminus. Both terminal structures are involved in genome protection and genome replication as well as regulation of the genome expression. Expression of viral proteins is through the production of a large polyprotein that is eventually cleaved by a set of viral proteases into 10 different viral proteins (Karasev and Gray 2013). PVY comprises a complex group of strains that have evolved over the years through genetic recombination and/or mutations which have adapted variably to different host cultivars and environments. The best known strains, naturally * Engy E. Abdel Aleem [email protected]; [email protected] 1
Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt
Botany and Microbiology Department Faculty of Science, Damanhour University, Damanhour, Egypt
2
infecting potatoes, are commonly separated into three groups according to their reaction on potato: P VYO group (ordinary or common strains) which generally induces severe systemic symptoms of crinkle, rugosity or leafdrop streak in potato; PVYN group (tobacco veinal necrosis strains) which produces very mild mottling in almost all potato cultivars; PVYC (stipple streak strains) which evokes a h
Data Loading...
