Isolation and Characterization of Serratiopeptidase Producing Bacteria from Mulberry Phyllosphere
- PDF / 2,664,196 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 26 Downloads / 246 Views
Isolation and Characterization of Serratiopeptidase Producing Bacteria from Mulberry Phyllosphere Diksha Koul1 · Devtulya Chander1,2 · Ravi S. Manhas1,2 · Asha Chaubey1,2 Received: 22 May 2020 / Accepted: 28 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Serratiopeptidase (EC 3.4.24.40), a proteolytic enzyme, is one of the most promising enzymes being used in biopharmaceutical industry. Mulberry phyllosphere, being an unexplored niche for exploration of protease production, was chosen for the present study. Protease producing bacteria were isolated from the tissues of mulberry plant as well as its rhizospheric soil. Two protease producing bacteria belonging to Serratia genus were found to be potential serratiopeptidase producers. Among them, the endophyte, i.e., Serratia marcescens MES-4 presented 95 Units/mL activity, while the soil isolate i.e., Serratia marcescens MRS-11 presented 156 Units/mL activity.
Introduction Serratiopeptidase (EC 3.4.24.40) is a proteolytic enzyme known to be produced from the non-pathogenic enterobacterium, Serratia sp. E-15. The serratiopeptidase produced by Serratia marcescens, as well as some other bacteria belonging to genus Serratia, is used as an anti-inflammatory drug [1, 2]. Serratiopeptidase has been reported to improve microcirculation and breaks down bradykinin, serotonin, and histamine which are responsible for the edema [1]. Serratiopeptidase is also reported for expectoration of sputum and for treatment of sinusitis, carpel tunnel syndrome, bronchitis, breast engorgement, etc. [2–4]. Serratia marcescens was first isolated from silk worm Bombyx mori L. [5]. Although Serratia marcescens has a wide range of distribution in nature, inhabiting soil, animals, as well as plants [1, 6], its presence in silk worm bears ecological importance. The serratiopeptidase secreted by these bacteria helps in dissolving the cocoon leading to the Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02280-0) contains supplementary material, which is available to authorized users. * Asha Chaubey [email protected] 1
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India
Academy of Scientific and Innovative Research, CSIR-Human Resource Development Centre, Campus Ghaziabad 201002, India
2
emergence of moth. Thus, isolates of Serratia sp. from its natural habitat i.e., mulberry phyllosphere may have higher potential for production of protease enzyme. The aim of the present study was to isolate bacteria from mulberry phyllosphere for their potential to produce serratiopeptidase. Although several reports for isolation of Serratia marcescens and serratiopeptidase have appeared [5–8], to the best of our knowledge, mulberry phyllosphere has not yet been explored for serratiopeptidase production. Therefore, we have made an attempt to explore the mulberry phyllosphere, particularly microorganisms associated with mulberry plant and its
Data Loading...
