Label-Free Semiquantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics Analysis of Laryngeal/Hypopharynge
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Label-Free Semiquantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics Analysis of Laryngeal/Hypopharyngeal Squamous Cell Carcinoma on Formalin-Fixed, Paraffin-Embedded Tissue Samples - a Pilot Study Andras Burian 1 & Laszlo Lujber 1 & Imre Gerlinger 1 & Tamas Jarai 2 & Eva Orosz 1 & Lilla Turiak 3 & Andras Acs 3,4 & Zoltan Hegedus 5,6 & Aniko Konigne Peter 7 & Tamas Tornoczki 8 & Katalin Gombos 9 & Laszlo Mark 6,10 Received: 25 July 2019 / Accepted: 11 June 2020 # The Author(s) 2020
Abstract Squamous cell carcinoma (SCC) of the head and neck region is the sixth most frequent malignancy with high mortality rate. Due to its poor prognosis it is considered a growing public health problem worldwide inspite of existing treatment modalities. Thus, early diagnosis of new diseases and recurrences is emerging on one hand, but on the other hand troublesome in the lack of reliable tumor markers in this field. The rapid development of proteomics has opened new perspectives in tumor marker discovery. Liquid chromatography/mass spectrometry (LC/MS) as the gold standard in proteomics enables the semi-quantitative analysis of proteins within various tissues. Abundance differences between tumor and normal tissue also can be interpreted as tumor specific changes. The aim of this study was to identify potential tumor markers of laryngeal/hypopharyngeal SCC by revealing abundance changes between cancerous and the surrounding phenotypically healthy tissue. After separating the phenotypically cancerous and healthy parts of formalin-fixed paraffin-embedded tissues, each sample underwent protein recovery process and tryptic digestion for label-free semi-quantitative LC/MS analysis. Eight proteins showed significantly higher abundance in tumor including tenascin, transmembrane emp24 domain-containing protein 2, cytoplasmic dynein light chain 1, coactosin-like protein, small proline-rich protein 2D, nucleolin, U5 small nuclear RNP 200-kDa helicase and fatty aldehyde dehydrogenase. Desmoglein-1 and keratin type I cytoskeletal 9 were down-regulated in tumor. Using Ingenuity Pathway Analysis we mapped the signaling pathways these proteins play role in regarding other tumors. Based on these findings these proteins may serve as promising biomarkers in the fight against laryngeal/hypopharyngeal SCCs. Keywords Biomarker discovery . Laryngeal cancer . LC/MS . Squamous cell carcinoma . Proteomics
* Laszlo Mark [email protected]
5
Biological Research Centre of the Hungarian Academy of Sciences, Institute of Biophysics, Temesvari Blvd 62, Szeged H-6726, Hungary
1
Clinical Centre, Department of Otorhinolaryngology and Head and Neck Surgery, University of Pecs, Munkacsy M Str 2, Pecs H-7621, Hungary
6
Medical School, Institute of Biochemistry and Medical Chemistry, University of Pecs, Szigeti Str 12, Pecs H-7624, Hungary
7
2
Tolna County Balassa Janos Hospital, Beri Balogh Adam Str 5-7, Szekszard H-7100, Hungary
Medical School, Institute of Bioanalysis, University of Pecs, Honved Str 1, Pecs H-7624,
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