Lipid Signaling Protocols
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellu
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Mark Waugh Editor
Lipid Signaling Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Lipid Signaling Protocols Edited by
Mark Waugh University College London, School of Life and Medical Sciences, London, United Kingdom
Editor Mark Waugh University College London School of Life and Medical Sciences London, United Kingdom
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3169-9 ISBN 978-1-4939-3170-5 (eBook) DOI 10.1007/978-1-4939-3170-5 Library of Congress Control Number: 2015947784 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Lipids are of central importance in the regulation of many aspects of cell function including receptor signaling, vesicle trafficking, and motility. There are a very large number of therapeutically important G-protein-coupled receptors and receptor tyrosine kinases that activate lipid signaling pathways and a range of diseases including some cancers and neurodegenerative conditions that are associated with defective intracellular lipid metabolism. Furthermore, as our knowledge of the molecular basis of human genetic disease expands so too does the long list of rare but often devastating inherited diseases that arise from mutations in genes encoding enzymes involved in lipid signaling. Therefore, there is a requirement for sensitive, reliable, and quantitative laboratory methods to investigate this medically important area of biochemistry. However, mainly because of their characteristic insolubility in aqueous buffers, lipids have collectively p
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