Liver Macrophages Stimulate the Expression of Hepatocyte Nuclear Factor-6 and Promote Hepatocyte Proliferation at the Ea
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Bulletin of Experimental Biology and Medicine, Vol. 170, No. 1, November, 2020
MICROBIOLOGY AND IMMUNOLOGY Liver Macrophages Stimulate the Expression of Hepatocyte Nuclear Factor-6 and Promote Hepatocyte Proliferation at the Early Stage of Liver Regeneration Y. Ch. Peng1, T. H. Lv2, Zh. K. Du1, X. N. Cun3, and K. M. Yang1
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 7, pp. 50-56, July, 2020 Original article submitted January 17, 2020 Hepatocyte nuclear factor (HNF-6) is a liver-specific protein and a key component in the differentiation process during the development of mature liver. The immunohistochemical staining and RT-PCR techniques were employed to examine the expression of HNF-6 and proliferation of Ki-67+ cells during the early regeneration of the liver on postsurgery in 3, 6, 12, and 24 h in original model of partial hepatectomy in rats. The earliest proliferating (Ki67+) cells were observed in 3 h after surgery in liver sinusoids (liver macrophages) and then in liver parenchyma. Expression of HNF-6 in hepatocytes and epithelial cells of the bile ducts attained maximum in 6 h after surgery. At later terms, this parameter somewhat decreased, but still surpassed the control level. Key Words: hepatocyte nuclear factor 6; liver regeneration; partial hepatectomy; cell proliferation
Liver diseases seriously threaten human health. Endstage liver diseases, especially liver cirrhosis, represent a worldwide health problem [15]. Liver cancer is the fifth most common malignancy and the third most common cause of cancer-related deaths. Five hundred million individuals are infected with hepatitis B or C viruses and a considerable part will progress to liver failure. In China, the incidence of hepatitis C and liver cancer-related mortality are high. Globally, about 53% new liver cancer cases are recorded in mainland China. In China, liver cancer is the second cancer-related cause of deaths. Along with the development of the liver transplant technology, stem cell-based strategies 1 Department of Anatomy, School of Basic Medical Science, Dali University, Dali, PR China; 2Department of Surgery of Dali Prefecture People’s Hospital, Dali, PR China; 3Department of Surgery of Affiliated Hospital of Dali University, Dali, PR China. Address for correspondence: [email protected]. K. M. Yang
are intensively studied as an attractive alternative approach to liver repair and regeneration. Liver regeneration is a complex process, and many genes and cell factors are involved in the dynamic control of liver regeneration [2,12]. Despite intensive molecular and cellular biology research, including hormone, growth factors and cytokine genes, the molecular mechanisms of liver regeneration, especially regulation mechanisms of this process remain poorly understood. The hepatocyte nuclear factor 6 (HNF-6) is a cellspecific transcription factor regulating gene expression in the liver. It was demonstrated using the chromatin immunoprecipitation technique that HNF-6 is involved in the regulation of cell proli
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