Mapping of quantitative trait loci for oil content in cottonseed kernel
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RESEARCH ARTICLE
Mapping of quantitative trait loci for oil content in cottonseed kernel QUAMPAH ALFRED† , HAI YING LIU† , HAI MING XU, JIN RONG LI, JIAN GUO WU, SHUI JIN ZHU∗ and CHUN HAI SHI∗ Department of Agronomy, Zhejiang University, Hangzhou 310058, People’s Republic of China
Abstract Oil content in cottonseed is a major quality trait which when improved through breeding could enhance the competitiveness of cottonseed oil among other vegetable oils. Cottonseed oil content is a quantitative trait controlled by genes in the tetraploid embryo and tetraploid maternal plant genomes, and the knowledge of quantitative trait loci (QTLs) and the genetic effects related to oil content in both genomes could facilitate the improvement in its quality and quantity. However, till date, QTL mapping and genetic analysis related to this trait in cotton have only been conducted in the tetraploid embryo genome. In the current experiment, an IF2 population of cottonseed kernels from the random crossing of 188 intraspecific recombinant inbred lines which were derived from the hybrid of two parents, HS46 and MARCABUCAG8US-1-88, were used to simultaneously locate QTLs for oil content in the embryo and maternal plant genomes. The four QTLs found to be associated with oil content in cottonseed were: qOC-18-1 on chromosome 18; qOC-LG-11 on linkage group 11; qOC-18-2 on chromosome 18; and qOC-22 on chromosome 22. At a high selection threshold of 0.05, there was strong evidence linking the QTLs above the oil content in cottonseed. Embryo additive and dominant effects from the tetraploid embryo genome, as well as maternal additive effects from the tetraploid maternal plant genome were found to be significant contributors to genetic variation in cottonseed oil content. [Alfred Q., Liu H. Y., Xu H. M., Li J. R., Wu J. G., Zhu S. J. and Shi C. H. 2012 Mapping of quantitative trait loci for oil content in cottonseed kernel. J. Genet. 91, 289–295]
Introduction Cottonseed is the second major product from the cotton plant (next to fiber) which serves as raw material for oil extraction or animal feed production (Ashokkumar and Ravikesavan 2008; Hamilton et al. 2004). It belongs to the group of unsaturated vegetable oil seeds that include safflower, corn, soybean, rapeseed and sunflower (Bert et al. 2003). The five largest cottonseed producing countries (China, 29%; United States, 19.9%; India, 14.2%; Pakistan, 9.5% and Brazil, 5%) currently acount for 73.1% of global output (Song and Zhang 2007). Its fatty acid profile generally consists of 70% unsaturated and 30% saturated fatty acids. Because of its low-cost and flavor stability compared to olive oil or canola, it has become increasingly acceptable in a wide range of processed foods, including bread and snack such as potato chips (Dowd et al. 2010). Cottonseed oil is however under scrutiny by some nutritionists for having too high-saturated fat and too ∗ For correspondence. E-mail: Chun Hai Shi, [email protected]; Shui Jin Zhu, [email protected]. † These authors contributed equally to this work.
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