Mesenchymal Stem Cell Assays and Applications
Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Mesenchymal Stem Cell Assays and Applications Edited by
Mohan C. Vemuri Stem Cells and Regenerative Medicine, Life Technologies, Frederick, MD, USA
Lucas G. Chase Cellular Dynamics International, Inc., Madison, WI, USA
Mahendra S. Rao Stem Cells and Regenerative Medicine, Life Technologies, Frederick, MD, USA
Editors Mohan C. Vemuri, Ph.D. Stem Cells and Regenerative Medicine Life Technologies Frederick, MD, USA [email protected]
Lucas G. Chase, Ph.D. Cellular Dynamics International, Inc. 525 Science Drive, Suite 200 Madison, WI, USA [email protected]
Mahendra S. Rao Stem Cells and Regenerative Medicine Life Technologies Frederick, MD, USA [email protected]
Please note that additional material for this book can be downloaded from http://extras.springer.com ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-998-7 e-ISBN 978-1-60761-999-4 DOI 10.1007/978-1-60761-999-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011922525 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Mesenchymal stem cells (marrow-derived stromal cells – MSC) were first harvested from the marrow well over two decades ago and were shown to likely play diverse functions in vitro and in vivo. MSC are thought to contribute to the stem cell niche in the marrow; contribute to smooth muscle, adipocyte, bone and cartilage development and repair; and in general contribute to the parenchyma of the most tissues and organs. MSC or MSC-like cells can therefore be isolated from a variety of tissues, and while subtle differences between these populations have been identified, their proper
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