Metagenomics Combined with Stable Isotope Probe (SIP) for the Discovery of Novel Dehalogenases Producing Bacteria
- PDF / 885,656 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 12 Downloads / 167 Views
FOCUSED REVIEW
Metagenomics Combined with Stable Isotope Probe (SIP) for the Discovery of Novel Dehalogenases Producing Bacteria Hemen Sarma1 · Sanket J. Joshi2 Received: 30 June 2020 / Accepted: 16 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation applications. Therefore, the identification of possible bacterial strains that produce dehalogenase is of great importance. Soil microorganisms that are regularly exposed to halogenated pesticides are a major source of hydrolytic dehalogenase. Their proper identification may be useful in the production of high-quality dehalogenase. DNA stable isotope probing (DNA-SIP) is quite a useful technique for the identification of active microorganisms that assimilate specific carbon substrates and nutrients. Metagenomics combined with a stable isotope probe (SIP) technique could therefore be used to detect bacterial dehalogenases in pesticides exposed agricultural soil. Keywords Halogenated compounds · Hydrolytic dehalogenases · Microbial bioremediation · DNA stable isotope probing (DNA-SIP) The largest groups of emerging environmental contaminants are halogenated organic compounds that are widely used to protect our agricultural crops such as herbicides, insecticides, fungicides and in the chemical industry as solvents, hydraulics and heat transfer fluids (Ang et al. 2018). The use and misuse of these hazardous chemicals is a major entry into the environment, resulting in undesirable conditions such as environmental pollution. Halogenated, natural and manufactured compounds are also commonly used in household products on a daily basis and therefore constitute one of the most important groups of environmental contaminants (Negri et al. 2007; DePierre 2003; Bidmanova et al. 2010; Elangovan et al. 2019). Halo-organic chemicals are also used for chemical synthesis and added to synthetic resins to promote plasticity and durability, and to reduce fragility. These * Hemen Sarma [email protected] * Sanket J. Joshi [email protected] 1
Department of Botany, N. N. Saikia College, Titabar, Assam 785630, India
Oil & Gas Research Center, Central Analytical and Applied Research Unit, Sultan Qaboos University, Muscat, Oman
2
halogenated compounds are widely used in public health applications, particularly in the process of water disinfection, from developed to economically marginal countries around the world (Islam et al. 2020). Aliphatic halogenated hydrocarbons that are introduced into the environment and that are already present in drinking water at significant levels are of serious concern (Islam et al. 2020). The widespread use of pesticides in agriculture has recently resulted in se
Data Loading...
