Microbial Extraction of Berberine from Phellodendron for Simultaneous Product Purification and Waste Resource Utilizatio
- PDF / 985,135 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 76 Downloads / 206 Views
RESEARCH ARTICLE
Microbial Extraction of Berberine from Phellodendron for Simultaneous Product Purification and Waste Resource Utilization Ying Li1 • Jinqing Wei1 • Xizhen Ge1
Received: 22 January 2019 / Revised: 8 September 2019 / Accepted: 27 September 2019 The National Academy of Sciences, India 2019
Abstract Berberine (BBR) is a broad-spectrum antimicrobial agent extracted from plants. However, traditional extraction of BBR depends on the massive consumption of organic solvent, leading to serious environmental pollutions. Here, the authors established a novel microbial method for BBR extraction from pulverized Phellodendron. A microbial consortium consisting of three Bacillus bacteria was constructed based on cellulase enzyme activities. The three bacteria together showed high cellulase enzyme activities for plant cell wall degradation. Moreover, no BBR degradation was detected during the cultivation of each strain. After the cultivation of this Bacillus consortium and pulverized Phellodendron for 56 h, BBR was successfully extracted into the medium. More importantly, the by-products of extraction such as phellodendrine were efficiently dissimilated by bacteria during the extraction, and the peak area of BBR occupied more than 60% of the total in the end of fermentation. The determination of extraction waste indicated that less than 10% of BBR remained in the extraction residue. Finally, the extraction residue that contains Bacillus consortium
Significance statement The authors established a novel microbial method for BBR extraction from pulverized Phellodendron by using the constructed Bacillus consortium. BBR was efficiently extracted from plant cell and some of the byproducts such as phellodendrine were consumed during extraction, leading to high BBR purity. More importantly, the extraction waste was applied for soil bacterial enrichment, and bacteria belonging to Bacillus were colonized in the soil. & Ying Li [email protected] 1
Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing 100023, China
was applied as fertilizer used in improving soil bacterial diversity. After the cultivation for 9 days, the amount of Bacillus genus in the soil was almost kept the same with initiation, meaning that Bacillus was colonized in the experimental soil. Overall, this is the first report on microbial extraction of chemicals from the plant, and this work provided a new way for resource re-utilization in the traditional Chinese medicine extraction industry. Keywords Berberine Microbial extraction Microbial consortium Bacillus Resource re-utilization
Introduction Berberine (BBR, CAS: 2086-83-1) is a kind of plant isoquinoline alkaloid found in root and rhizome of Hydrastis canadensis, Coptidis rhizome and Phellodendron [1, 2]. As a commonly used traditional Chinese medicine, BBR had been successfully applied to deal with intestinal infections [3]. As an effective drug for restriction cell growth of bacteria and fungi, BBR is also beco
Data Loading...
