MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth facto
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RESEARCH
Cellular & Molecular Biology Letters
Open Access
MicroRNA‑296‑5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor‑β‑induced epithelial–mesenchymal transition Meihui Chen1,2†, Chen Chen1†, Haiqing Luo3†, Jing Ren1, Qiuqin Dai1, Wenjia Hu1, Keyuan Zhou1, Xudong Tang1* and Xiangyong Li1* *Correspondence: [email protected]; [email protected] † Meihui Chen, Chen Chen and Haiqing Luo contributed equally to this manuscript. 1 Institute of Biochemistry and Molecular Biology of Guangdong Medical University, No. 2 Wenming Dong Road, Xiashan District, Zhanjiang 524023, Guangdong, China Full list of author information is available at the end of the article
Abstract Aim: To explore the effect of miR-296-5p on the metastasis of nasopharyngeal carcinoma (NPC) cells and investigate the underlying mechanism. Methods: The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. An miR-296-5p mimic and inhibitor were transfected into NPC cells. Then, immunofluorescence imaging, scratch wound-healing, transwell migration and invasion assays were used to observe the effects of miR-296-5p on cell metastasis and invasion. Real-time PCR and western blotting were carried out to detect the expressions of genes and proteins related to epithelial–mesenchymal transition (EMT). A dual luciferase reporter assay was used to identify whether TGF-β is the target gene of miR-296-5p. Finally, TGF-β expression plasmids were transfected into NPC cells to verify the role of TGF-β in the miR-296-5p-mediated inhibition of nasopharyngeal carcinoma cell metastasis. Results: Our results show that miR-296-5p inhibits the migratory and invasive capacities of NPC cells by targeting TGF-β, which suppresses EMT. Importantly, the miR296-5p level was significantly lower in human NPC tissues than in adjacent normal tissues. It also negatively correlated with TGF-β and was significantly associated with the lymph node metastasis of patients with NPC. Conclusions: Our findings show that miR-296-5p represses the EMT-related metastasis of NPC by targeting TGF-β. This provides new insight into the role of miR-296-5p in regulating NPC metastasis and invasiveness. Keywords: miR-296-5p, Metastasis, Invasion, EMT, Nasopharyngeal carcinoma
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