miR164-targeted TaPSK5 encodes a phytosulfokine precursor that regulates root growth and yield traits in common wheat (
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miR164-targeted TaPSK5 encodes a phytosulfokine precursor that regulates root growth and yield traits in common wheat (Triticum aestivum L.) Yuke Geng1,2 · Chao Jian1 · Wu Xu1 · Hong Liu1 · Chenyang Hao1 · Jian Hou1 · Hongxia Liu1 · Xueyong Zhang1 · Tian Li1 Received: 4 March 2020 / Accepted: 27 August 2020 © Springer Nature B.V. 2020
Abstract Key message TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. Abstract MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about the roles of less conserved miRNA-targeted genes in plants. In the current study, we identified TaPSK5, encoding a phytosulfokine precursor, as a novel target of miR164. Compared with miR164-targeted NAC transcription factor genes, TaPSK5 is less conserved between monocots and dicots. Expression analysis indicated that TaPSK5 homoeologs were constitutively expressed in wheat tissues, especially young spikes. Overexpression of TaPSK5-D and miR164-resistant TaPSK5-D (r-TaPSK5-D) led to increased primary root growth and grain yield in rice, with the latter having more significant effects. Comparison of the transcriptome between wild-type and r-TaPSK5-D overexpression plants revealed multiple differentially expressed genes involved in hormone signaling, transcription regulation, and reactive oxygen species (ROS) homeostasis. Moreover, we identified three TaPSK5-A haplotypes (TaPSK5-A-Hap1/2/3) and two TaPSK5B haplotypes (TaPSK5-B-Hap1/2) in core collections of Chinese wheat. Both TaPSK5-A-Hap1 and TaPSK5-B-Hap2 are favorable haplotypes associated with superior yield traits that were under positive selection during wheat breeding. Together, our findings identify miR164-targeted TaPSK5 as a regulator of root growth and yield traits in common wheat with potential applications for the genetic improvement of crops. Keywords miR164 · TaPSK5 · Root growth · Yield traits · Haplotype analysis · Wheat
Yuke Geng and Chao Jian contributed equally to this article. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01064-1) contains supplementary material, which is available to authorized users. * Xueyong Zhang [email protected]
Chenyang Hao [email protected]
* Tian Li [email protected]
Jian Hou [email protected]
Yuke Geng [email protected]
Hongxia Liu [email protected]
Chao Jian [email protected]
1
Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
College of Life and Environmental Science, Minzu University of China, Beijing 10081, China
Wu Xu [email protected] Hong Liu [email protected]
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Introduction MicroRNAs (miRNAs) are a class of abundant endogenous single-stranded small RNAs (~ 21 nt) produced from noncoding, imperfectly complementar
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