miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer
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RESEARCH
Open Access
miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer Elena A. Pudova1*, George S. Krasnov1, Kirill M. Nyushko2, Anastasiya A. Kobelyatskaya1, Maria V. Savvateeva1, Andrey A. Poloznikov2, Daniyar R. Dolotkazin2, Kseniya M. Klimina3, Zulfiya G. Guvatova1, Sergey A. Simanovsky4, Nataliya S. Gladysh5, Artemy T. Tokarev5, Nataliya V. Melnikova1, Alexey A. Dmitriev1, Boris Y. Alekseev2, Andrey D. Kaprin2, Marina V. Kiseleva2, Anastasiya V. Snezhkina1 and Anna V. Kudryavtseva1 From 11th International Young Scientists School “Systems Biology and Bioinformatics” – SBB-2019 Novosibirsk, Russia. 24-28 June 2019
Abstract Background: Prostate cancer is one of the most common and socially significant cancers among men. The aim of our study was to reveal changes in miRNA expression profiles associated with lymphatic dissemination in prostate cancer and to identify the most prominent miRNAs as potential prognostic markers for future studies. Methods: High-throughput miRNA sequencing was performed for 44 prostate cancer specimens taken from Russian patients, with and without lymphatic dissemination (N1 – 20 samples; N0 – 24 samples). Results: We found at least 18 microRNAs with differential expression between N0 and N1 sample groups: miR-1825p, miR-183-5p, miR-96-5p, miR-25-3p, miR-93-5p, miR-7-5p, miR-615-3p, miR-10b, miR-1248 (N1-miRs; elevated expression in N1 cohort; p < 0.05); miR-1271-5p, miR-184, miR-222-3p, miR-221-5p, miR-221-3p, miR-455-3p, miR-143-5p, miR-181c-3p and miR-455-5p (N0-miRs; elevated expression in N0; p < 0.05). The expression levels of N1-miRs were highly correlated between each other (the same is applied for N0-miRs) and the expression levels of N0-miRs and N1-miRs were anti-correlated. The tumor samples can be divided into two groups depending on the expression ratio between N0-miRs and N1-miRs. Conclusions: We found the miRNA expression signature associated with lymphatic dissemination, in particular on the Russian patient cohort. Many of these miRNAs are well-known players in either oncogenic transformation or tumor suppression. Further experimental studies with extended sampling are required to validate these results. Keywords: Prostate cancer, microRNA, Lymphatic dissemination, miRNA-Seq, Prognostic markers
* Correspondence: [email protected] 1 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in
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