Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rose
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Molecular isolation and characterization of translationally controlled tumor protein (TCTP) gene from Macrobrachium rosenbergii Phongthana Pasookhush 1,3 & Akapon Vaniksampanna 1,4 & Paisarn Sithigorngul 1,2 & Siwaporn Longyant 1,2 & Parin Chaivisuthangkura 1,2 Received: 27 January 2020 / Accepted: 29 June 2020/ # Springer Nature Switzerland AG 2020
Abstract
Translationally controlled tumor protein (TCTP) or fortilin is a highly conserved protein among eukaryotes and has important biological roles including anti-apoptotic activity which involves the elimination of virus-infected cells. In this study, the full-length cDNA of Macrobrachium rosenbergii TCTP (MrTCTP) was isolated. The cDNA sequence consisted of 783 bp with a 507-bp open reading frame encoding 168 amino acids. MrTCTP demonstrated all major characteristics of TCTP which include (1) highly homologous to TCTP protein family, (2) carrying two TCTP signatures, and (3) contained highly conserved amino acid sequences. The predicted structure of MrTCTP also revealed typical structure of TCTP which had three major domains including ßstranded core domain, helical domain, and flexible loop. The predicted molecular mass of MrTCTP was 18.94 kDa and sequence displays 83.93 and 78.57% identity to that of Eriocheir sinensis TCTP and Fenneropenaeus chinensis TCTP, respectively. Phylogenetic analysis revealed that the deduced amino acid sequence of MrTCTP was clustered into the invertebrate branch along with the sequences from arthropods and crustaceans. The mRNA expression showed that MrTCTP expressed in every tissue examined, preferentially in hepatopancreas and muscle. In addition, MrTCTP expression in the muscle was upregulated at 1 to 4 days after infection with M. rosenbergii nodavirus (MrNV). Finally, silencing of MrTCTP increased mortality rates post-MrNV challenge. Taken together, the study shows that MrTCTP may play an important role in shrimp immune response against MrNV infection. Keywords Translationally controlled tumor protein . Fortilin . TCTP . Macrobrachium rosenbergii . Macrobrachium rosenbergii nodavirus . MrNV . RNA interference . Innate immunity
* Parin Chaivisuthangkura [email protected] Extended author information available on the last page of the article
Aquaculture International
Introduction Crustacean immunity is solely relied on innate immune system which consists of humoral and cellular immune responses. Pattern recognition receptors (PRRs) such as Toll receptors recognize pathogens or pathogen-associated molecular patterns (PAMPs) and lead to the activation of humoral and cellular immune responses (Li and Xiang 2013). Apoptosis or programmed cell death is a cellular process for removing harmful cell that having uncontrolled proliferation or carrying genetic damage. Apoptosis is also considered as an important cellular response that limits viral replication and eliminate virally infected cells in multicellular organisms (Everett and McFadden 1999; Koyama et al. 2000). In crustacean, there are several proteins that have been reported to
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