Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria
- PDF / 297,539 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 81 Downloads / 167 Views
ORGINAL PAPER
Monitoring of gene expression in Fibrobacter succinogenes S85 under the co‑culture with non‑fibrolytic ruminal bacteria Naoki M. Fukuma · Satoshi Koike · Yasuo Kobayashi
Received: 18 July 2014 / Revised: 22 September 2014 / Accepted: 13 October 2014 / Published online: 30 October 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract Fibrobacter succinogenes is one of the most pivotal fibrolytic bacterial species in the rumen. In a previous study, we confirmed enhancement of fiber digestion in a co-culture of F. succinogenes S85 with non-fibrolytic ruminal strains R-25 and/or Selenomonas ruminantium S137. In the present study, mRNA expression level of selected functional genes in the genome of F. succinogenes S85 was monitored by real-time RT-PCR. Growth profile of F. succinogenes S85 was similar in both the monoculture and co-cultures with non-fibrolytics. However, expression of 16S rRNA gene of F. succinogenes S85 in the co-culture was higher (P
Data Loading...
