Nodulation of Retama species by members of the genus Microvirga in Morocco
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Nodulation of Retama species by members of the genus Microvirga in Morocco Lamrabet Mouad 1 & Lamin Hanane 1 & Bouhnik Omar 1 & Bennis Meryeme 1 & Abdelmoumen Hanaa 1 & Eulogio J. Bedmar 2 & Missbah El Idrissi Mustapha 1 Received: 20 June 2020 / Accepted: 18 September 2020 # Springer Nature B.V. 2020
Abstract The genetic diversity of bacterial populations’ nodulating Retama sphaerocarpa grown in the soils of Maamora cork forest (Morocco) was examined. ERIC-PCR fingerprinting of 30 strains distributed them in 2 groups, of which a representative strain from each group was studied by multilocus sequence analysis of the 16S rRNA, atpD, and gyrB genes. The two representative strains RSM25 and RSM32, grouped with “Microvirga tunisiensis”. This is the first description of Retama nodule bacteria as members of the genus Microvirga. A nodC-based phylogeny confirmed that the two representative strains RSM25 and RSM32 are affiliated with symbiovar mediterranense. The 2 strains were capable of nodulating not only R. sphaerocarpa but also R. monosperma, R. dasycarpa and L. luteus, and unable to nodulate Phaseolus vulgaris, Vachellia gummifera, Cicer arietinum, Vigna unguiculata and Glycine max. The inoculation of R. sphaerocarpa with RSM25 or RSM32 produced a 1.22-, and 1.36fold increase in the dry weight of the plants compared to those grown in the presence of 0.05% KNO3. The 2 strains used monosaccharides and disaccharides as the sole C source, but fructose, glucose, galactose, arabinose and starch were not used. They were unable to grow on glycine as a N source. Phosphate solubilization and siderophore production was not detected, but IAA or IAA-related compounds were produced. The alkaline pH of the sampling site soil in the Maamora forest where Retama grows could explain why Microvirga was the dominant species in the root nodules of the plants. Keywords Maamora Forest . Retama . Microvirga . Bradyrhizobium, symbiosis . Phylogeny
1 Introduction Plants of the genus Retama are wild shrubby legumes distributed throughout the Mediterranean area, more precisely in southwest Europe and northwest Africa (Zohary 1959; LeónGonzález et al. 2018). The genus Retama belongs to the Genisteae tribe of the Leguminosae (Fabaceae) family and contains four described species, R. monosperma, R. sphaerocarpa, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13199-020-00725-5) contains supplementary material, which is available to authorized users. * Missbah El Idrissi Mustapha [email protected] 1
Centre de Biotechnologies Végétale et Microbienne, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University of Rabat, Rabat, Morocco
2
Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), E-419, 18080 Granada, Spain
R. raetam, and R. dasycarpa. The latter is endemic to Morocco, and R. sphaerocarpa is widespread in Southern Spain, Morocco, Algeria and Tunisia. The distribution of R. monosperma is
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