Optimization and validation of a highly sensitive method for determining glyphosate in human urine by solid-phase extrac
- PDF / 913,275 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 73 Downloads / 224 Views
(2020) 25:83
Environmental Health and Preventive Medicine
RESEARCH ARTICLE
Open Access
Optimization and validation of a highly sensitive method for determining glyphosate in human urine by solid-phase extraction and liquid chromatography with tandem mass spectrometry: a methodological study Hiroshi Nomura1†, Risa Hamada1†, Isao Saito1, Kunihiko Nakane2, Ritsuko Sawa3, Miwa Ukai3, Eiji Shibata4, Mitsuo Sato5, Michihiro Kamijima6 and Jun Ueyama1*
Abstract Background: Glyphosate and its salt formulations are nonselective herbicides that have been extensively used worldwide, both for residential and agricultural purposes. The possible carcinogenicity and teratogenicity of glyphosate remain to be elucidated. We developed a sensitive and high-throughput analytical method for urinary glyphosate using liquid chromatography-tandem mass spectrometry with the aim of contributing to glyphosate exposure assessment in epidemiological studies. Methods: After urine dilution (creatinine matching dilution to 0.05 g creatinine/L), glyphosate was extracted using two types of solid phase extraction columns (SCX and NH2) with automated sample preparation instruments. The eluate was dried and dissolved in the mobile phase, followed by liquid chromatography-tandem mass spectrometry analysis. The optimized method was applied to urine samples obtained from 54 Japanese adults and children. Results: The results from the validation study demonstrated good recoveries (91.0-99.6%), within- and between-run precisions (< 15%), low detection limits (0.1 μg/L), and lower limit of quantification (0.3 μg/L). The detection frequency and median concentration of the urinary glyphosate in Japanese subjects were 59% and 0.25 μg/L (0.34 μg/g creatinine). Conclusions: Our reliable determination method was successful in measuring urinary glyphosate concentration. Moreover, this is the first biomonitoring report of urinary glyphosate levels in the Japanese general population. Keywords: Biomonitoring, Glyphosate, Human urine, LC-MS/MS, Solid-phase extraction
* Correspondence: [email protected] † Hiroshi Nomura and Risa Hamada contributed equally to this work. 1 Department of Biomolecular Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi 461-8673, Japan Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is no
Data Loading...
