PbrPOE21 inhibits pear pollen tube growth in vitro by altering apical reactive oxygen species content
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ORIGINAL ARTICLE
PbrPOE21 inhibits pear pollen tube growth in vitro by altering apical reactive oxygen species content Ming Qian1 · Linlin Xu1 · Chao Tang1 · Hao Zhang1 · Hongru Gao1 · Peng Cao1 · Hao Yin1 · Lei Wu1 · Juyou Wu1 · Chao Gu1 · Shaoling Zhang1 Received: 25 April 2020 / Accepted: 21 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion Genome-wide identification, tissue-specific expression analysis and functional characterization of selected genes containing the pear Pollen Olea europaea I domain reveal their roles in pollen tube growth. Abstract Genes containing the Pollen Olea europaea I (POE) domain play crucial roles in diverse growth and developmental processes. Nevertheless, the specific functions of POE family members in progression of pollen tube growth (PTG) remain uncharacterized. We identified 45 PbrPOE genes in the pear (Pyrus bretschneideri) genome, clustered into seven subclasses. PbrPOE genes contained 1 to 11 exons and 0 to 10 introns, with exon/intron structure mostly conserved within each subclass. Whole-genome duplication has mainly contributed to the duplication pattern of PbrPOE genes in pear. Expression profiles of 45 PbrPOE genes in 12 different pear tissues revealed that six PbrPOE genes (PbrPOE6, 12, 21, 29, 35 and 41) of subclass B were highly expressed during the growth of the pear pollen tube in vitro. PbrPOE21 was selected for further functional analysis on the basis of its high and differential expression pattern in pollen. Antisense oligodeoxynucleotide assays demonstrated that PTG was augmented in vitro when PbrPOE21 expression was significantly inhibited. Moreover, pollen tube length in vitro was reduced when PbrPOE21 was transitorily over-expressed using particle bombardment technology. Exogenous PbrPOE21 recombinant protein inhibited PTG in vitro at an optimum concentration of 1.8 µM. PbrPOE21 also affected reactive oxygen species content in the pear pollen tube apex. We suggest that PbrPOE21 inhibits PTG in vitro by altering apical reactive oxygen species content. Keywords Autocrine protein · Function · Gene family · PbrPOE21 · Pear · Pollen ole e I · Pollen tube growth · ROS Abbreviations POE Pollen Olea europaea I as-ODN Antisense oligodeoxynucleotide ROS Reactive oxygen species
Communicated by Dorothea Bartels. Ming Qian and Linlin Xu have contributed equally to this work Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03446-7) contains supplementary material, which is available to authorized users. * Shaoling Zhang [email protected] 1
College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
SI Self-incompatibility PTG Pollen tube growth
Introduction Pear is one of the most economically important and nutritious fruits and is thus favored worldwide. Self-incompatibility (SI) restricts development of the pear industry as it causes extra production costs asso
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