Production of hexaploid triticale by a synthetic hexaploid wheat-rye hybrid method
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Production of hexaploid triticale by a synthetic hexaploid wheat-rye hybrid method Ming Hao • Jiangtao Luo • Lianquan Zhang • Zhongwei Yuan Youwei Yang • Meng Wu • Wenjie Chen • Youliang Zheng • Huaigang Zhang • Dengcai Liu
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Received: 27 January 2013 / Accepted: 20 April 2013 / Published online: 30 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Hexaploid triticale, including its primary and secondary forms, is an important forage crop and a promising energy plant. Primary forms are usually developed by crossing Triticum turgidum L. with rye, with secondary forms obtained by crossing primary hexaploid triticale and/or hexaploid wheat with octoploid triticale. In this study, we developed an effective method for production of hexaploid triticale via hybridization of synthetic hexaploid wheat (SHW) with rye. The three employed SHW lines were derived from hybridization of T. turgidum with Aegilops tauschii Cosson, and inherited meiotic restitution genes, which can promote the formation of functional gametes in haploid status, from their T. turgidum
Ming Hao and Jiangtao Luo contributed equally to this work M. Hao J. Luo L. Zhang Z. Yuan Y. Yang M. Wu Y. Zheng D. Liu Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang 611130, Sichuan, People’s Republic of China
parents. Although the resulting tetraploid F1 hybrids with rye (genome ABDR) produced amphiploids (octoploid triticale) and partial amphiploids, the final hybrid products obtained through fertility selection over several generations were hexaploids. These hexaploids were the result of preferential elimination of D-genome chromosomes. In addition to complete hexaploid triticale with 28 intact A/B and 14 intact R chromosomes, we obtained hexaploid triticales with other chromosome constitutions, including monosomic, substitution, and translocation lines. Chromosomes 2D and 5D from the wild species A. tauschii were incorporated into the hexaploid triticales. Out of eight analyzed stable lines derived from three different SHW-L1/rye F1 plants, we observed four lines with small-fragment translocations between wheat and rye chromosomes. Rapid production of hexaploid W. Chen H. Zhang D. Liu (&) Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, People’s Republic of China e-mail: [email protected]
Present Address: Y. Yang College of Life Science, Hunan University of Arts and Science, Changde 415000, Hunan, People’s Republic of China Present Address: M. Wu Department of Botany, Miami University, 316 Pearson Hall, Oxford, OH 45056, USA
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triticales using this method involves two factors: (1) hybridization between hexaploid wheat with a meiotic restitution gene(s) and rye and (2) selection for good fertility during F3 and subsequent generations. Keywords Chromosome elimination Meiotic restitution Translocation Triticale
Introduction Triticale (9 Triticosecale Wittmack) is the first manmade cro
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