Qingchang Huashi granule ameliorates experimental colitis via restoring the dendritic cell-mediated Th17/Treg balance
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(2020) 20:291
RESEARCH ARTICLE
BMC Complementary Medicine and Therapies
Open Access
Qingchang Huashi granule ameliorates experimental colitis via restoring the dendritic cell-mediated Th17/Treg balance Jia Jia1†, Kai Zheng2*† , Hong Shen2, Jiangyi Yu1, Ping Zhu3, Shihai Yan4, Yi Xu2, Lei Zhu2, Yuelin Lu2, Peiqing Gu2 and Wan Feng2
Abstract Background: The balance between T helper 17 (Th17) cells and regulatory T cells (Tregs) is involved in immunological tolerance. Destruction of immunological tolerance by dendritic cell (DC)-mediated T cells is involved in the pathogenesis of ulcerative colitis (UC). Qingchang Huashi granule (QCHS) has been confirmed in the treatment of UC involved by inhibiting the activation of DCs. The aim of this study was to investigate the mechanism through which QCHS restores the Th17/Treg balance by modulating DCs in the treatment of UC. Methods: The effects of QCHS on Th17 cells, Tregs and DCs were detected in a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model. Furthermore, we injected QCHS-treated DCs into colitis model to test whether QCHS modulates the Th17/Treg balance via DCs. Tregs and Th17 cells were analyzed by FACS. IL-10, IL-17, and Foxp3 were measured by ELISA, Western blot and qRT-PCR. Results: Both QCHS and QCHS-treated DCs improved colonic histopathology, diminished Th17 cell differentiation and inhibited IL-17 production while promoting CD4+CD25+Foxp3+ Treg differentiation and augmenting IL-10 and Foxp3 expression in colitis mice. Additionally, QCHS reduced CD86 and MHC-II expression on DCs, decreased IL-12 production ex vivo and restored the Th17/Treg ratio in the colitis model. Conclusion: The findings of this study indicate that QCHS ameliorates TNBS-induced colitis by restoring the DCmediated Th17/Treg balance. Keywords: Ulcerative colitis, Chinese medicine, T helper 17, Regulatory T cells, Dendritic cells, Immunological tolerance
Background Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease (IBD) characterized by chronic relapse and a remitting clinical course [1]. IBD has become a global disease in the past few decades. The prevalence of UC is increasing in developed countries, although the * Correspondence: [email protected] † Jia Jia and Kai Zheng contributed equally to this work. 2 Department of Gastroenterology, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155 Hanzhong Road, Nanjing 210029, China Full list of author information is available at the end of the article
incidence is stable [2]. The prevalence and incidence of UC are lower in the Asia-Pacific region than in the West, but the incidence of UC in newly industrialized countries has increased steeply, especially in many parts of the Asia-Pacific region [3]. Important symptoms of UC include bloody diarrhea, mucous, urgency, tenesmus, abdominal cramping and weight loss. All of these symptoms impair health-related quality of life in patients and bring a substantial burden to these individuals [4]. IBD has been classified as a prototypically
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