The Antioxidant Rosmarinic Acid Ameliorates Oxidative Lung Damage in Experimental Allergic Asthma via Modulation of NADP
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ORIGINAL ARTICLE
The Antioxidant Rosmarinic Acid Ameliorates Oxidative Lung Damage in Experimental Allergic Asthma via Modulation of NADPH Oxidases and Antioxidant Enzymes Zhengmin Liang,1 Liqin Wu,1 Xin Deng,1 Qiuling Liang,1 Yangfeng Xu,1 Ruihan Deng,1 Li Lv,1 Min Ji,1 Zhihui Hao,2 and Jiakang He 1,3
Oxidative stress can induce lung damage and aggravate airway inflammation in asthma. Previously, we reported that rosmarinic acid (RA) exerted strong antiinflammatory effects in a mouse allergic asthma model. Therefore, we hypothesized that RA might also have antioxidative effects in a superimposed asthma mouse model with oxidative lung damage challenged with ovalbumin (Ova) and hydrogen peroxide (H2O2). We evaluated the antioxidative and anti-asthmatic activity of RA and explored its possible mechanisms of action. Mice sensitized to Ova and challenged with Ova and H2O2 were treated with RA 1 h after challenge. RA treatment greatly diminished the number of inflammatory cells; decreased IL-4, IL-5, and IL-13 production; increased IFN-γ secretion; significantly downregulated ROS production; and markedly upregulated the activities of SOD, GPx, and CAT. Furthermore, RA treatment resulted in a significant increase in the expression of Cu/Zn SOD and a notable reduction in NOX-2 and NOX-4 expression in lung tissues. These findings suggest that RA may effectively alleviate oxidative lung damage and airway inflammation in asthma.
Abstract—
Zhengmin Liang and Liqin Wu contributed equally to this work. 1
College of Animal Science and Technology, Guangxi University, Nanning, 530005, Guangxi, People’s Republic of China 2 The Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China 3 To whom correspondence should be addressed at College of Animal Science and Technology, Guangxi University, Nanning, 530005, Guangxi, People’s Republic of China. E-mail: [email protected] Abbreviation ROS, Reactive oxygen species; GPx, Glutathione peroxidase; SOD, Superoxide dismutase; CAT, Catalase; Cu/Zn SOD, Cuprozinc-superoxide dismutase; NOX, Nicotinamide adenine dinucleotide phosphate oxidase; BALF, Bronchoalveolar lavage fluid; Th, T helper; PBS, Phosphate-buffered saline; ELISA, Enzyme-linked immunosorbent assay; IL, Interleukin; RA, Rosmarinic acid; DEX, Dexamethasone; H&E, Hematoxylin and eosin; BALF, Bronchoalveolar lavage fluid
0360-3997/20/0000-0001/0 # 2020 Springer Science+Business Media, LLC, part of Springer Nature
Liang, Wu, Deng, Liang, Xu, Deng, Lv, Ji, Hao, and He KEY WORDS: rosmarinic acid; asthma; oxidative lung damage; antioxidation.
INTRODUCTION Rosmarinic acid (α-o-caffeoyl-3, 4dihydroxyphenyllactic acid, RA), a major active constituent of rosemary, was originally extracted from Rosmarinus officinalis by the Italian chemists Scarpati and Oriente in 1958 [1, 2]. Since then, RA has been used in the food and cosmetics industries for years based on its strong antioxidative properties [3, 4]. In addition, RA has obvious antiinf
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